Fluorescence-based Monitoring of the Ribosomal Activity to Optimize Yield from Recombinant Proteins

In vitro transcription/translation systems (ivtt) are used in biotechnology mainly to produce recombinant proteins whose production in vivo would be toxic. The experimental analysis normally involves autoradiography, i.e. it requires the labelling of translation products with radioactive isotopes. Handling these isotopes is expensive, a potential health hazard and limits throughput.

The present invention consists of stable bacterial strains with ribosomal subunits incorporating fluorescent markers, which have growth characteristics similar to wild type and which have an intact translation apparatus. This opens the opportunity for measuring the translation activity in real time using fluorescence. It is possible to carry out experiments in a shorter time and at less cost in multi-well plates, varying several reaction parameters in parallel to optimize yield.

Further information: PDF

Technologie-Lizenz-Büro (TLB) der Baden-Württembergischen Hochschulen GmbH
Phone: +49 (0)721/79 00 40

Contact
Dipl.-Biol. Marcus Lehnen, MBA

As Germany's association of technology- and patenttransfer agencies TechnologieAllianz e.V. is offering businesses access to the entire range of innovative research results of almost all German universities and numerous non-university research institutions. More than 2000 technology offers of 14 branches are beeing made accessable to businesses in order to assure your advance on the market. At www.technologieallianz.de a free, fast and non-bureaucratic access to all further offers of the German research landscape is offered to our members aiming to sucessfully transfer technologies.

Media Contact

info@technologieallianz.de TechnologieAllianz e.V.

All latest news from the category: Technology Offerings

Back to home

Comments (0)

Write a comment

Newest articles

Silicone sponge captures unknown bacteria

KIT researchers develop a chip that captures microbial dark matter in air, water, and soil – new tool for biotechnology and medicine. From human intestines to the bottom of the…

An exoplanet atmosphere as never seen before

The JWST just scored another first: a detailed molecular and chemical portrait of a distant world’s skies. The telescope’s array of highly sensitive instruments was trained on the atmosphere of…

AI tailors artificial DNA for future drug development

With the help of an AI, researchers at Chalmers University of Technology, Sweden, have succeeded in designing synthetic DNA that controls the cells’ protein production.  The technology can contribute to…

Partners & Sponsors