Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Inexpensive Enhancement of High-Resolution 3-D Fluorescence Microscopy: FAM Fluorescence Activation Microscopy

09.07.2013
Optical setups using two opposing micro-objectives have been propagated to enhance the axial resolution in 3-dimensional fluorescence microscopy using various illumination schemes. However, the coherent superposition of two counter propagating beams creates imaging artifacts, i.e. more or less pronounced side-maxima, which can only be effectively removed by arithmetic op-erations if their intensity is less than 50% of the main maximum.

Fluorescence Activation Microscopy (FAM) is a new alternative 3D fluorescence image technique besides the existing confocal or 2-photon excita-tion microscopy. In FAM, photo activatable dyes are used, so that improved axial resolution is achieved solely by the illumination beams in very much the same way than in 2-photon excitation and in contrast to the regular confocal case. In FAM, however, it is not relevant whether point, line or other structured illumination patterns are used as long as activation and excitation are applied simultaneously, permitting the use of devices such as micro lens arrays, birefringent devices, SLMs, LCD or DMD projectors, LED arrays, holographic pattern generators etc, so FAM enables optical sectioning without necessarily requiring confocal optics or 2-photon excitation. Together with fluorescent proteins such as DRONPA fast 3d life cell imaging also becomes possible. Calculated point spread functions (PSF) suggest that this method may perfect other high resolution imaging techniques such as 4Pi, STED and PALM additionally by introducing an axial resolution in the 70nm range.

Further Information: PDF

DKFZ (German Cancer Research Center, Deutsches Krebsforschungszentrum)
Phone: +49-6221-42 2955

Contact
Dr. Ruth Herzog

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=3231&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.

nachricht Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Lethal combination: Drug cocktail turns off the juice to cancer cells

A widely used diabetes medication combined with an antihypertensive drug specifically inhibits tumor growth – this was discovered by researchers from the University of Basel’s Biozentrum two years ago. In a follow-up study, recently published in “Cell Reports”, the scientists report that this drug cocktail induces cancer cell death by switching off their energy supply.

The widely used anti-diabetes drug metformin not only reduces blood sugar but also has an anti-cancer effect. However, the metformin dose commonly used in the...

Im Focus: New Foldable Drone Flies through Narrow Holes in Rescue Missions

A research team from the University of Zurich has developed a new drone that can retract its propeller arms in flight and make itself small to fit through narrow gaps and holes. This is particularly useful when searching for victims of natural disasters.

Inspecting a damaged building after an earthquake or during a fire is exactly the kind of job that human rescuers would like drones to do for them. A flying...

Im Focus: Topological material switched off and on for the first time

Key advance for future topological transistors

Over the last decade, there has been much excitement about the discovery, recognised by the Nobel Prize in Physics only two years ago, that there are two types...

Im Focus: Researchers develop method to transfer entire 2D circuits to any smooth surface

What if a sensor sensing a thing could be part of the thing itself? Rice University engineers believe they have a two-dimensional solution to do just that.

Rice engineers led by materials scientists Pulickel Ajayan and Jun Lou have developed a method to make atom-flat sensors that seamlessly integrate with devices...

Im Focus: Three components on one chip

Scientists at the University of Stuttgart and the Karlsruhe Institute of Technology (KIT) succeed in important further development on the way to quantum Computers.

Quantum computers one day should be able to solve certain computing problems much faster than a classical computer. One of the most promising approaches is...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

ICTM Conference 2019: Digitization emerges as an engineering trend for turbomachinery construction

12.12.2018 | Event News

New Plastics Economy Investor Forum - Meeting Point for Innovations

10.12.2018 | Event News

EGU 2019 meeting: Media registration now open

06.12.2018 | Event News

 
Latest News

New discoveries predict ability to forecast dementia from single molecule

12.12.2018 | Health and Medicine

CCNY-Yale researchers make shape shifting cell breakthrough

12.12.2018 | Physics and Astronomy

Pain: Perception and motor impulses arise in the brain independently of one another

12.12.2018 | Health and Medicine

VideoLinks
Science & Research
Overview of more VideoLinks >>>