In Vivo Screening Based on Fluorescence to Identify Novel Antimicrobial Substances

The hierarchical and precisely controlled process creating ribosomes in living cells is known as ribosome assembly and is relatively little researched. In the eyes of many experts the early processes in the creation of ribosomes offer attractive targets for antimicrobial agents. The systematic search for such substances is made more difficult by the fact, that currently no suitable screening processes exist.

The present invention consists of stable bacterial strains with ribosomal subunits incorporating fluorescent markers, which have growth characteristics similar to wild type, and which have an intact translation apparatus. The positioning of the fluorophores allows for disturbances in the ribosome assembly to be detected in vivo by a fluorescence-based readout process. The process has been optimized for use with multi-well plates and thus is suitable for use in high throughput screenings (HTS).

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