VisuVivo – A proliferation marker to visualise cell cycle progression in vitro and in vivo with high spatial resolution
The invention provides a nucleic acid expression construct encoding a fusion protein comprising a fluorescence reporter protein (like EGFP) and a protein with a wild-type destruction signal (like Anillin). Localised to subcellular structures during cell cycle progression, it presents a fluorescence marker for imaging cell cycle progression in vitro and in vivo.
Challenge: The cell cycle comprises consecutive phases termed G1, S (synthesis), G2 (interphase) and M (mitosis). Cells that temporarily or reversibly stop dividing enter quiescence, named the G0-phase. To differentiate between cells that start to divide again and resting cells is still an unreached goal. In addition, available cell cycle indicators are unable to distinguish between cell division and acytokinetic mitosis which is karyokinesis without cyotkinesis or endoreplication which is continuing rounds of DNA replication without karyokinesis.
Further Information: PDF
PROvendis GmbH
Phone: +49 (0)208/94105 10
Contact
Dipl.-Ing. Alfred Schillert
Media Contact
All latest news from the category: Technology Offerings
Newest articles
High-energy-density aqueous battery based on halogen multi-electron transfer
Traditional non-aqueous lithium-ion batteries have a high energy density, but their safety is compromised due to the flammable organic electrolytes they utilize. Aqueous batteries use water as the solvent for…
First-ever combined heart pump and pig kidney transplant
…gives new hope to patient with terminal illness. Surgeons at NYU Langone Health performed the first-ever combined mechanical heart pump and gene-edited pig kidney transplant surgery in a 54-year-old woman…
Biophysics: Testing how well biomarkers work
LMU researchers have developed a method to determine how reliably target proteins can be labeled using super-resolution fluorescence microscopy. Modern microscopy techniques make it possible to examine the inner workings…
