VisuVivo – A proliferation marker to visualise cell cycle progression in vitro and in vivo with high spatial resolution

The invention provides a nucleic acid expression construct encoding a fusion protein comprising a fluorescence reporter protein (like EGFP) and a protein with a wild-type destruction signal (like Anillin). Localised to subcellular structures during cell cycle progression, it presents a fluorescence marker for imaging cell cycle progression in vitro and in vivo.

Challenge: The cell cycle comprises consecutive phases termed G1, S (synthesis), G2 (interphase) and M (mitosis). Cells that temporarily or reversibly stop dividing enter quiescence, named the G0-phase. To differentiate between cells that start to divide again and resting cells is still an unreached goal. In addition, available cell cycle indicators are unable to distinguish between cell division and acytokinetic mitosis which is karyokinesis without cyotkinesis or endoreplication which is continuing rounds of DNA replication without karyokinesis.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Dipl.-Ing. Alfred Schillert

Media Contact TechnologieAllianz e.V.

All latest news from the category: Technology Offerings

Back to home

Comments (0)

Write a comment

Newest articles

Neurons: How RNA granules grow and shrink

LMU scientists have shown that small aggregates function as temporary RNA repositories, which are regulated by neural activity. Cells constantly have to adapt the level of activity of certain genes…

Custom ‘headphones’ boost atomic radio reception 100-fold

Researchers at the National Institute of Standards and Technology (NIST) have boosted the sensitivity of their atomic radio receiver a hundredfold by enclosing the small glass cylinder of cesium atoms…

New tech aims to drive down costs of hydrogen fuel

Researchers from North Carolina State University have developed a new technique for extracting hydrogen gas from liquid carriers which is faster, less expensive and more energy efficient than previous approaches….

Partners & Sponsors