Highly specific nucleases for gene targeting and gene therapy

The new chimeric nucleases exhibit an increased preference and specificity for DNA cleavage sites composed of two or three recognition sites and allow single cuts in the genome which reduces the toxic side effects substantially.
The high specificity and preference of the new chimeric nucleases is based on a fusion of TALE proteins as DNA binding modules with a highly specific restriction endonuclease, PvuII, as DNA cleavage module.

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