Many biological processes depend on oligomeric protein-protein interactions (PPI). However, state-of-the-art in vivo PPI techniques focus on analysing binary interactions (i.e. the Split-Ubiquitin System (SUS)1). The SUS can also be used to analyse binding of three proteins in the so-called SUS Bridge Assay (SUB)2 see Figure A. Nevertheless, this assay makes an unbiased screening approach cumbersome and its low efficiency restricts the identification of meaningful candidates. Here, we present the first screening system in yeast that permits high-throughput screening of cDNA libraries for ternary binding partners of a known interaction couple. This is achieved by a unique combination of SUB and yeast mating. Technical prerequisites are special 2in1-vectors3, which allow simultaneous transformation of Bait I and Bait II on a single plasmid in yeast of one mating type and the cDNA library in the other see Figure B.
Further information: PDF
Eberhard Karls Universität Tübingen
Phone: +49 (7071) 29-72639
Dr. Rolf Hecker
As Germany's association of technology- and patenttransfer agencies TechnologieAllianz e.V. is offering businesses access to the entire range of innovative research results of almost all German universities and numerous non-university research institutions. More than 2000 technology offers of 14 branches are beeing made accessable to businesses in order to assure your advance on the market. At www.technologieallianz.de a free, fast and non-bureaucratic access to all further offers of the German research landscape is offered to our members aiming to sucessfully transfer technologies.
email@example.com | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly
The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
14.11.2018 | Life Sciences
14.11.2018 | Life Sciences
14.11.2018 | Earth Sciences