DNA is not merely a carrier of genetic information; DNA is a useful building material for nanoscale structures. In a way similar to origami, the Japanese art of paper folding, a long single strand of DNA can be folded into nearly any three-dimensional shape desired with the use of short DNA fragments.
Various methods for binding proteins to DNA-origami structures have previously been developed, but in most cases they require modification of the protein. “A method based only on proteins is desirable,” says Morii, “because it would simplify and accelerate the binding of proteins to the origami.”
Morii and his team settled on the use of zinc-finger proteins as “adapters”. A polypeptide chain of zinc-finger protein grabs a zinc ion to form a stable compact fold; this fold referred to as a “zinc finger” and can bind to specific DNA patterns. It is possible to make zinc fingers that recognize any DNA pattern desired.
The scientists produced rectangular origami structures with several defined cavities. At these locations, the origamis contain various DNA-recognition patterns for different zinc fingers. The researchers then made proteins that contain zinc-finger units at one end and a fluorescing protein or biotin molecule at the other end. Biotin binds specifically to the large protein streptavidin. Atomic force microscopic images show that the streptavidin molecules always bind specifically to the intended cavity in the origami rectangle.
“Our results demonstrate that zinc fingers are suitable site-selective adapters for targeting specific locations within DNA-origami structures,” says Morii. “Several different adapters carrying different proteins can independently bind at defined locations on this type of nanostructure.”
Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201108199
Takashi Morii | Angewandte Chemie
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
17.08.2018 | Physics and Astronomy
17.08.2018 | Information Technology
17.08.2018 | Life Sciences