Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New procedure to obtain induced pluripotent stem cells

10.06.2015

A new protocol that simplifies the process that allows induced pluripotent stem cells was developed at the Center for Biomedicine of the European Academy of Bolzano. While the traditional methodology requires fresh blood, the new procedure allows cells from frozen blood samples to regress to a similar state to that of embryonic stem cells. The reprogrammed cells can be used to understand how some diseases develop and to test new therapies. The new protocol reduces costs and work time in the laboratory.

For their ability to differentiate into other cell types, the embryonic stem cells hold a large potential in the medical industry. Their use, however, poses ethical questions due to the fact that in order to obtain them, it is necessary to destroy the embryo. For this reason, medical researches use the so-called induced pluripotent stem cells (iPSC).


Research on iPS-cells at the EURAC laboratory

EURAC/Bortolotti

In fact, it is possible to reprogram adult cells that can be obtained by simply drawing blood and making them “regress” to a similar state to that of embryonic stem cells. The reprogrammed cells (iPSC) are capable of creating all the cell types of an adult organism, such as brain or heart cells.

The iPSC technology is revolutionizing medical science, allowing for the exploration of molecular mechanisms that regulate diseases, providing new therapeutic targets, and providing opportunities for the discovery of new drugs.

Thanks to the iPSC, the possibility to develop a truly personalized medicine will become more realistic, enabling the testing of specific drugs on cells, such as neurons and cardiomyocytes, otherwise impossible to isolate from patients with specific diseases.

“The procedure that we have developed simplifies the process that enables these cells to be obtained. With the traditional method, the blood is centrifuged with different reagents, capable of separating the blood cells according to their size. Our protocol, on the other hand, does not require the use of reagents.

This allows for the reduction of costs, time, and the complexity of procedures to be performed in the laboratory”, explain Viviana Meraviglia and Alessandra Zanon, researchers at EURAC Center for Biomedicine and main authors of the study.

“The big advantage of our method is that it can also be applied to blood samples previously collected and preserved in a biobank. We will be able to access samples collected in other studies that we have performed or from biobanks in other research centers”, continues Alessandra Rossini, study coordinator.
The research performed by the EURAC Center for Biomedicine focuses especially on cardiovascular and neurological diseases, such as Parkinson´s.

Currently, researchers are engaged in the differentiation of the cardiomyocyte induced pluripotent cells to study a genetic disease associated with a right ventricular arrhythmia (arrhythmogenic right ventricular dysplasia, ARVD) and in dopaminergic neurons, meaning specific brain cells, to study the development of Parkinson´s.

The study was published in the international scientific journal ”JoVE”, Journal of Visualized Experiments (http://www.jove.com/video/52885), who invited a troupe into the Center for Biomedicine laboratories to film all the steps of the new methodology in detail, so they could be replicated in other research centers.

Laura Defranceschi | idw - Informationsdienst Wissenschaft
Further information:
http://www.eurac.edu

More articles from Life Sciences:

nachricht Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY

nachricht NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

A smart safe rechargeable zinc ion battery based on sol-gel transition electrolytes

20.07.2018 | Power and Electrical Engineering

Reversing cause and effect is no trouble for quantum computers

20.07.2018 | Information Technology

Princeton-UPenn research team finds physics treasure hidden in a wallpaper pattern

20.07.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>