Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

TAPPIR® - Tunable Aqueous Polymer Phase Impregnated Resins

19.11.2012
Aqueous Two-Phase Extraction (ATPE) is a process proved efficient for the gentle purification of various biomolecules. Fast mass transfer rates and less damage to proteins that are sensitive to mechanical stresses and organic solvents render this unit operation an alternative to commonly used downstream processing technique.

However ATPE has no large-scale industrial applications yet. This is based substantially on very long phase separation times resulting from low interfacial tensions and low density differences between the phases producing intensive and stable emulsions. This causes an increased footprint of the required mixer/settler devices or the application of additional equipment such as energy consuming centrifuges. The present invention relates to a process for separation or purification of biomolecules via ATPE. The advantages are maintained and the major limitation of long phase separation time is overcome by immobilizing one of the two phases in porous particles. Suspending the impregnated particles in the second aqueous phase containing the biomolecules creates the emulsion without any need for phase separation after the extraction step. During the separation process, the biomolecules partition between the aqueous phase surrounding the particles and the immobilized second aqueous phase inside of the particles’ pores.

The TAPPIR®-technology can be used on one hand for separation which means the isolation of biomolecules from e.g. fermentation broth (Capture), on the other hand for purification which means the removal of biomolecules like objectionable proteins, viruses, DNA, lipids or other cell debris from biocatalytic processes within the separation of metabolites (Clarification). Moreover the flexibility of the TAPPIR®-technology extends to different possible operation modes namely packed bed, fluidized bed or in situ for e.g. extractive fermentation. In a first example, BSA partitioning in a PEG/Citrate-TAPPIR®-system was characterized. The depletion of BSA in the aqueous salt phase could be increased by 10% in relation to conventional extraction methods. This means nearly a twofold higher efficiency by the use of a considerably faster separation process.

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Contact
Dipl.-Ing. Alfred Schillert

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=2557&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht Context-sensitive indexing of hemodynamic parameters
12.03.2015 | TechnologieAllianz e.V.

nachricht EMULSION BUSTER 2.0 - Easy Emulsion Separation in Bioprocesses
22.04.2015 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fast and Accurate 3-D Imaging Technique to Track Optically-Trapped Particles

KAIST researchers published an article on the development of a novel technique to precisely track the 3-D positions of optically-trapped particles having complicated geometry in high speed in the April 2015 issue of Optica.

Daejeon, Republic of Korea, April 23, 2015--Optical tweezers have been used as an invaluable tool for exerting micro-scale force on microscopic particles and...

Im Focus: NOAA, Tulane identify second possible specimen of 'pocket shark' ever found

Pocket sharks are among the world's rarest finds

A very small and rare species of shark is swimming its way through scientific literature. But don't worry, the chances of this inches-long vertebrate biting...

Im Focus: Drexel materials scientists putting a new spin on computing memory

Ever since computers have been small enough to be fixtures on desks and laps, their central processing has functioned something like an atomic Etch A Sketch, with electromagnetic fields pushing data bits into place to encode data.

Unfortunately, the same drawbacks and perils of the mechanical sketch board have been just as pervasive in computing: making a change often requires starting...

Im Focus: Exploding stars help to understand thunderclouds on Earth

How is lightning initiated in thunderclouds? This is difficult to answer - how do you measure electric fields inside large, dangerously charged clouds? It was discovered, more or less by coincidence, that cosmic rays provide suitable probes to measure electric fields within thunderclouds. This surprising finding is published in Physical Review Letters on April 24th. The measurements were performed with the LOFAR radio telescope located in the Netherlands.

How is lightning initiated in thunderclouds? This is difficult to answer - how do you measure electric fields inside large, dangerously charged clouds? It was...

Im Focus: On the trail of a trace gas

Max Planck researcher Buhalqem Mamtimin determines how much nitrogen oxide is released into the atmosphere from agriculturally used oases.

In order to make statements about current and future air pollution, scientists use models which simulate the Earth’s atmosphere. A lot of information such as...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

HHL Energy Conference on May 11/12, 2015: Students Discuss about Decentralized Energy

23.04.2015 | Event News

“Developing our cities, preserving our planet”: Nobel Laureates gather for the first time in Asia

23.04.2015 | Event News

HHL's Entrepreneurship Conference on FinTech

13.04.2015 | Event News

 
Latest News

Electrons Move Like Light in Three-Dimensional Solid

24.04.2015 | Materials Sciences

Connecting Three Atomic Layers Puts Semiconducting Science on Its Edge

24.04.2015 | Materials Sciences

Understanding the Body’s Response to Worms and Allergies

24.04.2015 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>