Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

“PCGP” - Polymer-Conjugate Technology for the Rapid Chemoenzymatic Synthesis of Glycopeptides

19.11.2012
Glycosylation is known to play a key role in numerous biological and biochemical functions such as, e.g. protein folding, cell-cell communication, cellular differentiation, cell-matrix interaction and viral invasion. Moreover, the glycosylation pattern of a peptide may have a great impact on the immunogenicity and the proteolytic stability of said peptide. A glycopeptide itself and/or a carbohydrate moiety cleaved off said glycopeptide may serve as an epitope.

So far, glycopeptides are mostly obtained from natural sources. However, purification of said peptides is highly challenging and laborious. The glycopeptides found in nature are mostly proteins of more than 100 amino acids in length, often comprising more than one glycosylation site(s) and their glycosylation pattern cannot be fully controlled. Therefore, there is still the need for efficient methods for producing glycopeptides by synthetical means. Currently, there are two common strategies for the chemical synthesis of glycopeptides known in the art. According to the first strategy, a peptide strand is synthesized and, during the synthesis of said peptide, one or more glycosylated amino acid building block(s) are integrated in the sequence instead of standard amino acids. According to the second strategy, a peptide is first fully synthesized, then, subsequently, one or more protecting groups are cleaved off selectively and the carbohydrate building block is conjugated to the peptide. Both strategies bear several severe drawbacks. The present invention relates to a method for the production of glycosylated peptides conjugated with one or more hydrophilic polymer(s) and glycosylating the polymer-conjugated peptide by means of one or more glycosyltransferase(s).

Further Information: PDF

PROvendis GmbH
Phone: +49 (0)208/94105 10

Contact
Dipl.-Ing. Alfred Schillert

info@technologieallianz.de | TechnologieAllianz e.V.
Further information:
http://www.technologieallianz.de/angebote.php?sort=sag&id=2656&lang=en
http://www.technologieallianz.de

More articles from Technology Offerings:

nachricht Highly specific nucleases for gene targeting and gene therapy
01.07.2015 | TechnologieAllianz e.V.

nachricht New Furin Inhibitors for Treatment of Infectious Diseases
01.07.2015 | TechnologieAllianz e.V.

All articles from Technology Offerings >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Viaducts with wind turbines, the new renewable energy source

Wind turbines could be installed under some of the biggest bridges on the road network to produce electricity. So it is confirmed by calculations carried out by a European researchers team, that have taken a viaduct in the Canary Islands as a reference. This concept could be applied in heavily built-up territories or natural areas with new constructions limitations.

The Juncal Viaduct, in Gran Canaria, has served as a reference for Spanish and British researchers to verify that the wind blowing between the pillars on this...

Im Focus: X-rays and electrons join forces to map catalytic reactions in real-time

New technique combines electron microscopy and synchrotron X-rays to track chemical reactions under real operating conditions

A new technique pioneered at the U.S. Department of Energy's Brookhaven National Laboratory reveals atomic-scale changes during catalytic reactions in real...

Im Focus: Iron: A biological element?

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and a half billion years ago.

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and...

Im Focus: Thousands of Droplets for Diagnostics

Researchers develop new method enabling DNA molecules to be counted in just 30 minutes

A team of scientists including PhD student Friedrich Schuler from the Laboratory of MEMS Applications at the Department of Microsystems Engineering (IMTEK) of...

Im Focus: Bionic eye clinical trial results show long-term safety, efficacy vision-restoring implant

Patients using Argus II experienced significant improvement in visual function and quality of life

The three-year clinical trial results of the retinal implant popularly known as the "bionic eye," have proven the long-term efficacy, safety and reliability of...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

World Conference on Regenerative Medicine in Leipzig: Last chance to submit abstracts until 2 July

25.06.2015 | Event News

World Conference on Regenerative Medicine: Abstract Submission has been extended to 24 June

16.06.2015 | Event News

MUSE hosting Europe’s largest science communication conference

11.06.2015 | Event News

 
Latest News

Veja Mate Offshore orders 67 wind turbines including record long-term service

06.07.2015 | Press release

The quantum middle man

06.07.2015 | Physics and Astronomy

Southampton researchers go with the flow to help protect endangered European eel

06.07.2015 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>