By identifying a 30-year-old mistaken assumption, Johns Hopkins Kimmel Cancer Center scientists have found that substituting a simple bleach solution for more complex tools makes a DNA separation technique called electrophoresis five times faster and less costly.
Reported in the February issue of BioTechniques, the scientists say that using the compound sodium boric acid in DNA electrophoresis may speed genetic discoveries. The scientists searched old literature and dozens of compounds to find one that could replace antiquated solutions used to conduct the electric current necessary to separate negatively charged DNA molecules, the building blocks of genetic code. In electrophoresis, DNA is fed through porous, jellylike slabs of sugar (also known as a DNA gel) to reveal the outlines of the code, with small DNA molecules crawling up the gel faster than less mobile larger particles as the current passes through.
Like the two poles on a battery, the difference in the positive and negative charges represents a voltage. "DNA just needs to know its in a voltage and it will move," says Scott Kern, M.D., professor of oncology and pathology at the Johns Hopkins Kimmel Cancer Center. "So, the most important feature of a solution in electrophoresis is its ability to carry a voltage."
Vanessa Wasta | JHMI
Fingerprint' technique spots frog populations at risk from pollution
27.03.2017 | Lancaster University
Parallel computation provides deeper insight into brain function
27.03.2017 | Okinawa Institute of Science and Technology (OIST) Graduate University
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
27.03.2017 | Earth Sciences
27.03.2017 | Life Sciences
27.03.2017 | Life Sciences