By identifying a 30-year-old mistaken assumption, Johns Hopkins Kimmel Cancer Center scientists have found that substituting a simple bleach solution for more complex tools makes a DNA separation technique called electrophoresis five times faster and less costly.
Reported in the February issue of BioTechniques, the scientists say that using the compound sodium boric acid in DNA electrophoresis may speed genetic discoveries. The scientists searched old literature and dozens of compounds to find one that could replace antiquated solutions used to conduct the electric current necessary to separate negatively charged DNA molecules, the building blocks of genetic code. In electrophoresis, DNA is fed through porous, jellylike slabs of sugar (also known as a DNA gel) to reveal the outlines of the code, with small DNA molecules crawling up the gel faster than less mobile larger particles as the current passes through.
Like the two poles on a battery, the difference in the positive and negative charges represents a voltage. "DNA just needs to know its in a voltage and it will move," says Scott Kern, M.D., professor of oncology and pathology at the Johns Hopkins Kimmel Cancer Center. "So, the most important feature of a solution in electrophoresis is its ability to carry a voltage."
Vanessa Wasta | JHMI
Oestrogen regulates pathological changes of bones via bone lining cells
28.07.2017 | Veterinärmedizinische Universität Wien
Programming cells with computer-like logic
27.07.2017 | Wyss Institute for Biologically Inspired Engineering at Harvard
Spectrally narrow x-ray pulses may be “sharpened” by purely mechanical means. This sounds surprisingly, but a team of theoretical and experimental physicists developed and realized such a method. It is based on fast motions, precisely synchronized with the pulses, of a target interacting with the x-ray light. Thereby, photons are redistributed within the x-ray pulse to the desired spectral region.
A team of theoretical physicists from the MPI for Nuclear Physics (MPIK) in Heidelberg has developed a novel method to intensify the spectrally broad x-ray...
Physicists working with researcher Oriol Romero-Isart devised a new simple scheme to theoretically generate arbitrarily short and focused electromagnetic fields. This new tool could be used for precise sensing and in microscopy.
Microwaves, heat radiation, light and X-radiation are examples for electromagnetic waves. Many applications require to focus the electromagnetic fields to...
Strong light-matter coupling in these semiconducting tubes may hold the key to electrically pumped lasers
Light-matter quasi-particles can be generated electrically in semiconducting carbon nanotubes. Material scientists and physicists from Heidelberg University...
Fraunhofer IPA has developed a proximity sensor made from silicone and carbon nanotubes (CNT) which detects objects and determines their position. The materials and printing process used mean that the sensor is extremely flexible, economical and can be used for large surfaces. Industry and research partners can use and further develop this innovation straight away.
At first glance, the proximity sensor appears to be nothing special: a thin, elastic layer of silicone onto which black square surfaces are printed, but these...
3-D shape acquisition using water displacement as the shape sensor for the reconstruction of complex objects
A global team of computer scientists and engineers have developed an innovative technique that more completely reconstructs challenging 3D objects. An ancient...
26.07.2017 | Event News
21.07.2017 | Event News
19.07.2017 | Event News
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28.07.2017 | Physics and Astronomy