Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Tilted acoustic tweezers separate cells gently

26.08.2014

Precise, gentle and efficient cell separation from a device the size of a cell phone may be possible thanks to tilt-angle standing surface acoustic waves, according to a team of engineers.

"For biological testing we often need to do cell separation before analysis," said Tony Jun Huang, professor of engineering science and mechanics. "But if the separation process affects the integrity of the cells, damages them in any way, the diagnosis often won't work well."


This is a schematic illustration of working principle and device structure for a tilted-angle standing surface acoustic wave-based cell-separation device.

Credit: Tony Huang, Penn State

Tilted-angle standing surface acoustic waves can separate cells using very small amounts of energy. Unlike conventional separation methods that centrifuge for 10 minutes at 3000 revolutions per minute, the surface acoustic waves can separate cells in a much gentler way.

The power intensity and frequency used in this study are similar to that used in ultrasonic imaging, which has proven to be extremely safe, even for fetuses. Also, each cell experiences the acoustic wave for only a fraction of a second, rather than 10 minutes.

... more about:
»MIT »acoustic »blood »efficiently »malaria »materials »waves

"The tilted-angle standing surface acoustic waves method has the least disturbance or disruption to the living cells being separated compared to other available methods so far," said Ming Dao, principal research scientist, materials science and engineering, Massachusetts Institute of Technology.

"It adds to the portfolio of latest technology developments for separating such things as rare circulating tumor cells in the blood."

Previous work by Huang showed that acoustic tweezers work by setting up a standing surface acoustic wave. If two sound sources are placed opposite each other and each emits the same wavelength of sound, there will be a location where the opposing sounds cancel each other. Because sound waves have pressure, they can push very small objects, so a cell or nanoparticle will move with the sound wave until it reaches the location where there is no longer movement.

If the sound sources are at right angles to each other, an evenly spaced set of rows and columns form in a checkerboard pattern. In this case, the team from Penn State, MIT and Carnegie Mellon University used simulation programs to determine the angle the sound sources should be tilted at to produce the best separation. They report their results today (Aug. 25) online in the Proceedings of the National Academies of Science.

By tilting the sound source so that it is not perpendicular, the researchers created better separation distance and could more efficiently sort cells.

The acoustic tweezers are made by manufacturing an interdigital transducer, which creates the sound, onto the piezoelectric chip surface. Standard photolithography creates microchannels in which the liquid containing the cells flow.

The researchers created the separator, which can run continuously. The device separated 9.9-micrometer particles from 7.3-micrometer particles so efficiently that 97 percent of the 7.3-micrometer particles went to the correct location. The device can also separate cancer cells from white blood cells with high efficiency and purity. It is simple and inexpensive to fabricate and does not need strict alignment to achieve this separation.

"The method we describe in this paper is a step forward in the detection and isolation of circulating tumor cells in the body," said Subra Suresh, one of the study's authors and president of Carnegie Mellon University. "It has the potential to offer a safe and effective new tool for cancer researchers, clinicians and patients."

The researchers see devices like this one separating cancer cells from other cells, bacteria from blood, white blood cells from red blood cells and malaria parasites from blood, to name a few uses.

###

Other Penn State researchers on this project were Xiaoyun Ding, graduate student and co-lead author; Sz-Chin Steven Lin, graduate student; Peng li, post doctoral fellow and Yuchao Chen, graduate student, engineering science and mechanics; and Sixing Li, graduate student, cell and developmental biology.

Other researchers were Zhangli Peng, former postdoctoral fellow, materials science and engineering, and Michela Geri, graduate student, mechanical engineering, both at MIT.

The National Institutes of Health and the National Science Foundation funded this work.

A'ndrea Elyse Messer | Eurek Alert!
Further information:
http://www.psu.edu

Further reports about: MIT acoustic blood efficiently malaria materials waves

More articles from Materials Sciences:

nachricht Ultrasonic Fingerprint Sensor May Take Smartphone Security to New Level
01.07.2015 | American Institute of Physics (AIP)

nachricht Physical study may give boost to hydrogen cars
01.07.2015 | American Institute of Physics

All articles from Materials Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: X-rays and electrons join forces to map catalytic reactions in real-time

New technique combines electron microscopy and synchrotron X-rays to track chemical reactions under real operating conditions

A new technique pioneered at the U.S. Department of Energy's Brookhaven National Laboratory reveals atomic-scale changes during catalytic reactions in real...

Im Focus: Iron: A biological element?

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and a half billion years ago.

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and...

Im Focus: Thousands of Droplets for Diagnostics

Researchers develop new method enabling DNA molecules to be counted in just 30 minutes

A team of scientists including PhD student Friedrich Schuler from the Laboratory of MEMS Applications at the Department of Microsystems Engineering (IMTEK) of...

Im Focus: Bionic eye clinical trial results show long-term safety, efficacy vision-restoring implant

Patients using Argus II experienced significant improvement in visual function and quality of life

The three-year clinical trial results of the retinal implant popularly known as the "bionic eye," have proven the long-term efficacy, safety and reliability of...

Im Focus: Lasers for Fast Internet in Space – Space Technology from Aachen

On June 23, the second Sentinel mission was launched from the space mission launch center in Kourou. A critical component of Aachen is on board. Researchers at the Fraunhofer Institute for Laser Technology ILT and Tesat-Spacecom have jointly developed the know-how for space-qualified laser components. For the Sentinel mission the diode laser pump module of the Laser Communication Terminal LCT was planned and constructed in Aachen in cooperation with the manufacturer of the LCT, Tesat-Spacecom, and the Ferdinand Braun Institute.

After eight years of preparation, in the early morning of June 23 the time had come: in Kourou in French Guiana, the European Space Agency launched the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

World Conference on Regenerative Medicine in Leipzig: Last chance to submit abstracts until 2 July

25.06.2015 | Event News

World Conference on Regenerative Medicine: Abstract Submission has been extended to 24 June

16.06.2015 | Event News

MUSE hosting Europe’s largest science communication conference

11.06.2015 | Event News

 
Latest News

Offshore wind park Westermost Rough officially inaugurated

01.07.2015 | Press release

Siemens Velaro train wins "Red Dot" award

01.07.2015 | Awards Funding

Liquids on Fibers - Slipping or Flowing?

01.07.2015 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>