Cell line for screening demethylating agents using an endogenous epigenetically silenced reporter
The invention provides a high-throughput screening system for agents that influence DNA methylation.
Using the Zinc Finger Nuclease (ZFN) technique, EGFP and G418 resistance genes were stably integrated in the genome of a human cell line under the control of an endogenous promoter. Since this promoter is methylated (epigenetically silenced), the reporter genes are not expressed. After the addition of demethylating agents GFP or G418 can be used as a readout in a screening assay for epigenetic reactivation. The system has a z-value of 0.75 using G418 as readout.
Further Information: PDF
Deutsches Krebsforschungszentrum DKFZ
Phone: +49-6221-42 2955
Contact
Dr. Ruth Herzog
Media Contact
All latest news from the category: Technology Offerings
Newest articles
FDmiX: Fast, robust series production of nanoparticles
Nucleic acid-based medications such as mRNA vaccines are opening up new therapeutic approaches. These active ingredients must be enclosed inside nanoparticles to ensure that they get to where they are…
Sensor measures oxygen content of breath
Oxygen saturation in the blood that is either too low or too high can cause physical harm or even death. This is why patients’ oxygen concentraions are monitored continuously in…
Laser immersion probe for smart inline monitoring of water and wastewater
A new type of laser-based immersion probe, which the Fraunhofer Institute for Laser Technology ILT in Aachen is testing as part of BMBF- and EU-funded projects with industrial partners and…
