Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

RUDN biochemists found out how ROS affect cisplatin resistance in ovarian cancer cells

14.09.2018

Long-term use of anti-cancer drugs treatment may lead to the development of multiple drug resistance reducing the efficiency of chemotherapy. Scientists are aware of several mechanisms for drug resistance development in tumor cells. They are to a great extent associated with the activation of proteins that get medicinal drugs out of cells, as well as with changes in the genes controlling cell survival and programmed cell death (apoptosis). One of the least studied mechanisms of drug resistance development is connected with the influence of reactive oxygen species (ROS) that are formed during cell life and can destroy organic molecules.

RUDN biochemists studied the mechanism of drug resistance development in ovarian cancer cells under treatment with cisplatin. The researchers found out that the process was connected with increased expression (production of proteins coded by DNA molecules) of genes of key antioxidant enzymes (i.e. destroying ROS):


The mechanism of oxidative stress.

Courtesy of Allen Dressen

Mn-superoxide dismutase, catalase, glutathione peroxidase-1, hemoxygenase-1, as well as reduced expression of the enzyme NADPH-oxidase, which is responsible for ROS generation in the cell.

In the normal state there is a balance between the formation and destruction of ROS. Any changes in this balance may cause damage in the internal structure of the cells leading to number of diseases including cancer.

On the other hand, high concentrations of ROS may also lead to the death of cancer cells. This effect is used in cancer therapy, and cisplatin may operate on a similar principle.

In their work the scientists used the real time RT-PCR method and western blotting to evaluate gene expression.

The discovered redox-dependent mechanism of drug resistance development shows how well tumor cells can adapt to negative environmental conditions. The mechanism helps healthy cells survive, but in case of malignant tumors hinders their treatment.

"We found that under treatment of the ovarian cancer cells with cisplatin a new mechanism is triggered, changing the balance between generation and elimination of ROS; it is called as the adaptive antioxidant response.

The studied mechanism of drug resistance development would help create new combined methods for ovarian cancer treatment," said Elena Kalinina, the author of the study, doctor of biological science, and professor of RUDN Institute of Medicine.

Media Contact

Valeriya V. Antonova
antonova_vv@rudn.university

http://eng.rudn.ru/ 

|
Further information:
http://dx.doi.org/10.1002/2211-5463.12453

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

Im Focus: Coping with errors in the quantum age

Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly

The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Epoxy compound gets a graphene bump

14.11.2018 | Materials Sciences

Microgel powder fights infection and helps wounds heal

14.11.2018 | Health and Medicine

How algae and carbon fibers could sustainably reduce the athmospheric carbon dioxide concentration

14.11.2018 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>