Researchers from the Ruhr-Universität Bochum (RUB) and from the MPI Dortmund have for the first time successfully reproduced the recycling process of proteins regulating cellular transport in a biophysical experiment. In doing so, they traced in detail the way the central switch protein Rab is being extracted from the lipid membrane.
The Rab protein (grey and magenta) with bound GDP (multi-coloured) sits on a membrane surface. As the infrared ray is reflected from the surface, the processes that take place on the membrane can be studied. The GDI, represented by the hand, seizes the Rab protein and extracts it from the membrane. The timeline of the infrared spectra (top centre) is resolved in the spectrometer (top right).
Credit: Konstantin Gavriljuk, RUB
The team of PD Dr Carsten Kötting, Prof Dr Klaus Gerwert (Department of Biophysics, RUB) and Prof Dr Roger S. Goody (Max Planck Institute for Molecular Physiology, Dortmund) has published the spectroscopic and dynamic data in the PNAS journal's Online Early Edition. "Until now, this protein's interactions have only ever been studied in a solution – i.e. without a lipid membrane. The step into the protein's natural environment opens up entirely new possibilities," says Carsten Kötting. This is because many disease-relevant protein interactions within a cell take place on a membrane.From solution to membrane
The project funds are supplied by SFB 642 "GTP and ATP-dependent Membrane Processes", whose spokesperson is Prof Gerwert.Bibliographic record
PD Dr Carsten Kötting, Department of Biophysics, Faculty of Biology and Biotechnology at the Ruhr-Universität, 44780 Bochum, Germany, tel. +49/234/32-24461, e-mail: Koetting@bph.rub.deA click away
Dr. Carsten Kötting | EurekAlert!
Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung
Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.
Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
16.11.2018 | Health and Medicine
16.11.2018 | Life Sciences
16.11.2018 | Life Sciences