Recombinant prion protein for high-throughput prion diagnosis
Due not least to the BSE-crisis in Great Britian and the new variant of Creutzfeldt-Jakob disease (vCJD) a great need for a reliable diagnostic method of prion proteins exists, especially for the discrimination of prion cases from other neurodegenerative diseases. The present invention provides a method for the purification of recombinant prion proteins (rPrP), which are suitable to be used in the currently accepted detection method (Real-time quaking induced conversion, RT-QuIC) and lead to faster, more specific and overall more reliable results. Therefore, a “ready-to use protein” is provided for the detection of prion protein in blood, brain and cerebrospinal fluid (CSF).
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