Controlled Activation of LINE-1 Retrotransposons in Mammals

</a><strong>Background</strong><br>

LINE-1 (Long INterspersed Element-1) elements represent the major class of mammalian non-LTR (long terminal repeat) retrotransposons which are cov-ering 35 % of the human genome. LINE-1 is the only autonomous non-LTR retrotransposon in the human genome because it is coding for the protein machinery required for its mobilization. <br><br> <strong>Technology</strong><br> We offer a system which allows inducible, controlled and conditional expres-sion and retrotransposition of non-LTR retrotransposons, in particular mam-malian LINE-1 elements, in mammalian systems (cell culture and in vivo). The LINE-1 element is tagged with a reporter gene cassette and set under control of a tetracycline/doxycycline-inducible promoter. The modified LINE-1 elements can be used for an efficient insertional mutagenesis screen in both germ line and somatic cells. Gene trapping approaches based on controlled retrotransposition during defined time periods of the embryonic development can be used to identify cancer genes in specific tissues and cell types. Inducible modified LINE-1 elements which are tagged with a therapeutic gene can be used as a gene delivery system in gene therapeutic approaches.<br><br> <b>Benefits:</b><ul> <li>Induction of L1 retrotransposition for a defined period of time</li> <li>Tissue-specific L1 retrotransposition induction possible by using a vector-construct, coding for rtTA or tTA functionally linked to a tissue-specific promoter </li> <li>Proof of principle Tet/Dox – On/Off system shown in M2-HeLa cells</li></ul> <p><strong>IP Rights</strong><br> Patent granted in DE, GB, FR (EP 2205750), US patent pending (priority date 10/2007). <br><br> <strong>Patent Owner</strong><br> Paul-Ehrlich-Institut, Federal Agency for Sera and Vaccines, Langen

Further Information: PDF

ipal GmbH
Phone: +49 (0)30/2125-4820

Contact
Dr. Dirk Dantz

Media Contact

info@technologieallianz.de TechnologieAllianz e.V.

All latest news from the category: Technology Offerings

Back to home

Comments (0)

Write a comment

Newest articles

Trotting robots reveal emergence of animal gait transitions

A four-legged robot trained with machine learning by EPFL researchers has learned to avoid falls by spontaneously switching between walking, trotting, and pronking – a milestone for roboticists as well…

Innovation promises to prevent power pole-top fires

Engineers in Australia have found a new way to make power-pole insulators resistant to fire and electrical sparking, promising to prevent dangerous pole-top fires and reduce blackouts. Pole-top fires pose…

Possible alternative to antibiotics produced by bacteria

Antibacterial substance from staphylococci discovered with new mechanism of action against natural competitors. Many bacteria produce substances to gain an advantage over competitors in their highly competitive natural environment. Researchers…

Partners & Sponsors