Nucleic Fluorescent Probes for the Specific Detection of Single Base Alterations

The present invention relates to forced intercalation probes (FIT-probes) based on nucleoside analogues with fluorescent artificial nucleobases. Thereby the nucleoside analogue is incorporated into DNA or RNA in the place of a single native base.<br>As such, FIT-probes may be employed in a large number of applications including genetic diagnostics, disease predisposition, pharmacogenetics and pathogen detection. The FIT-probes exhibit a simplistic mode of action and are able to detect single base alteration. They further possess few design constraints and show melting peak data which can be interpreted easily. The assay has been demonstrated to function efficiently directly from samples without prior purification of nucleic acids making the probe technology suitable for point-of-care diagnostics.</p>

<p><strong>Benefits</strong> <ul> <li>More specific and sensitive than common PNA probes</li> <li>First time manufacturing of DNA probes</li> <li>Abdication of linker enhances dye properties</li> <li>Enhanced sensitivity as probes differ between correct and incorrect hybridization</li> <li>Application at room temperature</li> <li>Wide range of application</li> <li>DNA probes allow detection in living cells by Fluorescence in situ hybridization (FISH)</li> <li>Enzyme activity is not influenced</li> <li>Inexpensive production</li> </ul> <p><strong>IP Rights</strong><br> An European application was filed on September 8th, 2009.<br> <br> <strong>Origin</strong><br> The technology was developed at the Humboldt-Universität Berlin, Germany.<br>  </p>

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