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Detection of relevant variants of the KRAS and the BRAF gene

Scientists of the Saarland University and the Charité Berlin established an assay which allows the selective detection of all 14 clinically relevant variants of the KRAS gene (codon 12 and 13) and the BRAF gene (codon 600-2). Compared to the most frequently used technique of direct sequencing, the assay shows a much higher sensitivity with a reliable detection limit of 5%, compared to 20% of mutated DNA in a wild type background, with causing only one third of the costs of direct sequencing, as the assay does not require complicated establishment procedures, expensive lab automation or technical replicates.

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Universität des Saarlandes Wissens- und Technologietransfer GmbH PatentVerwertungsAgentur der saarländischen Hochschulen
Phone: +49 (0)681/302-71302

Dipl.-Kfm. Axel Koch (MBA), Dr. Conny Clausen, Dr. Hauke Studier, Dr. Susanne Heiligenstein | TechnologieAllianz e.V.
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