What do you get when you mix RNA chips and zebrafish? A new two-stage method for studying human gene expression differs in human stem cells and progenitor cells, according to a new article in the open access journal PLoS Biology. In the paper, Catherine Verfaillie and colleagues identify 277 human genes with different gene expression profiles in stem and progenitor cells involved in blood cell development, or hematopoiesis. The paper also suggests that a developmental regulator called fibroblast growth factor may play a role in hematopoiesis.
Using microarray technology and zebrafish, researchers discovered which human genes are active during hematopoiesis, the formation of blood cells. (Photo: Verfaillie et al.)
The new method may also have relevance beyond the field of blood development. "This could be done not only for hematopoietic stem cells as is done here but also for brain, pancreas, endothelium, smooth and cardiac muscle, because of the high homology between man and fish in development of these tissues," explains Verfaillie.
The RNA transcript microarray, or "RNA chip," allowed Verfaillie and colleagues to compare gene expression in cell cultures with many or few hematopoietic stem cells. Previously, the authors had developed a method to harvest stem cells using bone marrow and blood from umbilical cords. Sixty-one of the 277 genes found to have different expressions profiles in stem and progenitor cells had no known function in human hematopoietic stem cells, though the genes do resemble genes found in zebrafish. Fish with mutated versions of fourteen of these genes developed blood disorders, suggesting that the genes are necessary for normal hematopoiesis. Three of the 14 genes are known to play a role in fibroblast growth factor signaling. In the future, researchers may learn the specific relationship between fibroblast growth factor and hematopoiesis.
Paul Ocampo | EurekAlert!
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences