There is increasing evidence suggesting that allergic-response diseases such as asthma, perennial rhinitis, and atopic dermatitis result from proteolytic or other enzymatic activity in common allergens. Dust is commonly allergenic, and to investigate the presence of active proteases in dust, researchers led by Jennifer Harris at The Scripps Research Institute and Nicolas Winssinger at the Université Louis Pasteur examined an extract derived from dust mites. The researchers devised and used a novel combined-library approach for this purpose--a method that enabled simultaneous identification of proteases from complex samples and isolation of protease-specific inhibitors.
In this way, Der 1 p was identified as an active cysteine protease in dust mite extracts, and a Der 1 p-specific small-molecule inhibitor was isolated from a peptide nucleic acid (PNA)-encoded small-molecule library. As a result of this investigation, Der 1 p was implicated in the cleavage of the CD4+ T cell receptor CD25--the Der 1 p inhibitor has a dose-dependent ability to protect the receptor on whole cells. This study demonstrates the utility of this new approach for quickly identifying active proteases involved in allergic responses or other disease processes and effective inhibitors that can be further developed for diagnostic or therapeutic research.
Nicolas Winssinger, Robert Damoiseaux, David C. Tully, Bernhard H. Geierstanger, Keith Burdick, and Jennifer L. Harris: "PNA-Encoded Protease Substrate Microarrays"
Heidi Hardman | EurekAlert!
Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo
Full of hot air and proud of it
18.04.2018 | University of Pittsburgh
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.
Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
19.04.2018 | Materials Sciences
19.04.2018 | Physics and Astronomy
19.04.2018 | Physics and Astronomy