There is increasing evidence suggesting that allergic-response diseases such as asthma, perennial rhinitis, and atopic dermatitis result from proteolytic or other enzymatic activity in common allergens. Dust is commonly allergenic, and to investigate the presence of active proteases in dust, researchers led by Jennifer Harris at The Scripps Research Institute and Nicolas Winssinger at the Université Louis Pasteur examined an extract derived from dust mites. The researchers devised and used a novel combined-library approach for this purpose--a method that enabled simultaneous identification of proteases from complex samples and isolation of protease-specific inhibitors.
In this way, Der 1 p was identified as an active cysteine protease in dust mite extracts, and a Der 1 p-specific small-molecule inhibitor was isolated from a peptide nucleic acid (PNA)-encoded small-molecule library. As a result of this investigation, Der 1 p was implicated in the cleavage of the CD4+ T cell receptor CD25--the Der 1 p inhibitor has a dose-dependent ability to protect the receptor on whole cells. This study demonstrates the utility of this new approach for quickly identifying active proteases involved in allergic responses or other disease processes and effective inhibitors that can be further developed for diagnostic or therapeutic research.
Nicolas Winssinger, Robert Damoiseaux, David C. Tully, Bernhard H. Geierstanger, Keith Burdick, and Jennifer L. Harris: "PNA-Encoded Protease Substrate Microarrays"
Heidi Hardman | EurekAlert!
The birth of a new protein
20.10.2017 | University of Arizona
Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
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20.10.2017 | Interdisciplinary Research