There is increasing evidence suggesting that allergic-response diseases such as asthma, perennial rhinitis, and atopic dermatitis result from proteolytic or other enzymatic activity in common allergens. Dust is commonly allergenic, and to investigate the presence of active proteases in dust, researchers led by Jennifer Harris at The Scripps Research Institute and Nicolas Winssinger at the Université Louis Pasteur examined an extract derived from dust mites. The researchers devised and used a novel combined-library approach for this purpose--a method that enabled simultaneous identification of proteases from complex samples and isolation of protease-specific inhibitors.
In this way, Der 1 p was identified as an active cysteine protease in dust mite extracts, and a Der 1 p-specific small-molecule inhibitor was isolated from a peptide nucleic acid (PNA)-encoded small-molecule library. As a result of this investigation, Der 1 p was implicated in the cleavage of the CD4+ T cell receptor CD25--the Der 1 p inhibitor has a dose-dependent ability to protect the receptor on whole cells. This study demonstrates the utility of this new approach for quickly identifying active proteases involved in allergic responses or other disease processes and effective inhibitors that can be further developed for diagnostic or therapeutic research.
Nicolas Winssinger, Robert Damoiseaux, David C. Tully, Bernhard H. Geierstanger, Keith Burdick, and Jennifer L. Harris: "PNA-Encoded Protease Substrate Microarrays"
Heidi Hardman | EurekAlert!
Enzyme with surprising dual function
24.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn
Flexibility and arrangement - the interaction of ribonucleic acid and water
24.01.2018 | Forschungsverbund Berlin e.V.
Physicists have developed a technique based on optical microscopy that can be used to create images of atoms on the nanoscale. In particular, the new method allows the imaging of quantum dots in a semiconductor chip. Together with colleagues from the University of Bochum, scientists from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute reported the findings in the journal Nature Photonics.
Microscopes allow us to see structures that are otherwise invisible to the human eye. However, conventional optical microscopes cannot be used to image...
On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.
We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...
What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...
For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.
Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...
At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.
No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...
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08.12.2017 | Event News
24.01.2018 | Physics and Astronomy
24.01.2018 | Health and Medicine
24.01.2018 | Health and Medicine