The graph shows the binding of an RGS-His fusion protein to immobilised antibodies against a tetra-His, a penta-His and an RGS-His epitope. All antibodies recognize the epitope but only the anti-RGS-His antibody shows no dissociation when bound to the RGS-fusion protein after an association time of 450 seconds.
A complete characterization of monoclonal antibodies also includes the determination of epitope specificity for a given set of monoclonal antibodies. Epitope mapping is a powerful tool in analysing the surface topography of an antigen. The binding of an antibody to the antigen defines a specific binding site or epitope which sterically interferes with the binding of another antibody which has the same or a closely located binding site. The specificity of pairs of antibodies can easily be determined by testing the simultaneous binding to the antigen. Dinstinct binding sites can be identified by binding of both antibodies in parallel whereas an identical or closely located binding site prevents binding of the second antibody. BIA technology (Biomolecular Interaction Analysis) is ideally suited to automatically test panels of monoclonal antibodies and define their epitope specificity pattern.
Our antibody service “eitope mapping” includes the generation of working plans for the experimental setup, the performance of the epitope mapping analysis on a Biacore instrument and the complete evaluation of the results documented in a written report with figures. This report provides you with information about the epitope specificity pattern for your set of monoclonal antibodies i.e. which antibodies bind to the same epitope and which can bind simultaneously because they have different binding sites on the antigen. Please enquire for a detailed quote concerning your specific problem.
Dr. Stephan Drewianka | BIAFFIN GmbH & Co KG
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Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
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In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
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