Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Just the Two Of Us

23.11.2011
Stable Dinucleotide–RNA Duplexes Show Promise in Biotechnology

Nucleic acid technology has revolutionized the field of biomedicine, as it can be effectively utilized in the diagnosis, treatment, and prevention of genetic diseases.

The efficacy of most oligonucleotide therapies is, however, limited as a result of the lability of oligonucleotides in biological fluids and, in particular, their poor delivery to the site of action. A Swedish team headed by R. Strömberg recently reported in the European Journal of Organic Chemistry that modification of oligonucleotides with a 2'-O-carbamoyl moiety greatly increases the stability of these compounds, which may render their use in constructs for biotechnological and therapeutic applications viable.

Efficiency in the regulation of gene expression is readily achieved if turnover of the target RNA is obtained, but this can only occur if native enzymes recognize the relevant oligonucleotide complex. The ability to catalytically cleave a specific sequence of RNA at a specific site is of high potential value in biotechnology and oligonucleotide therapy. Thus, the development of oligonucleotide-based artificial nucleases (OBANs) as artificial enzymes capable of cleaving mRNA sequences arising from genetic or viral diseases is highly sought.

In this context, the scientists set out to modify oligonucleotides with the judicious choice of a 2'-carbamoylmethyl (CM) moiety. Substitution at the 2-position was an important prerequisite, as this has been shown to lead to the formation of stable duplexes with the target RNA, and it was also believed that the CM moiety could further increase the stability of the duplex through hydrogen bonding.

The team was able to show that the 2'-O-carbamoyl modification substantially protected the dinucleotide against enzyme-catalyzed degradation by phosphodiesterase I and made it virtually resistant to degradation by phosphodiesterase II. This, together with the reported increased thermal stability of the duplexes, makes the often-neglected 2'-O-carbamoyl moiety an interesting modification in the pursuit of future compounds that may one day help in the treatment of genetic diseases.

Author: Roger Strömberg, Karolinska Institutet, Stockholm (Sweden), http://www.bionut.ki.se/groups/rst/
Title: Stability of a 2'-O-(Carbamoylmethyl)adenosine-Containing Dinucleotide
European Journal of Organic Chemistry, Permalink to the article: http://dx.doi.org/10.1002/ejoc.201101264

Roger Strömberg | Wiley-VCH
Further information:
http://www.wiley.com

Further reports about: CHEMISTRY Organic RNA genetic disease viral disease

More articles from Life Sciences:

nachricht Reptile vocalization is surprisingly flexible
30.05.2017 | Max-Planck-Institut für Ornithologie

nachricht New photocatalyst speeds up the conversion of carbon dioxide into chemical resources
29.05.2017 | DGIST (Daegu Gyeongbuk Institute of Science and Technology)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Strathclyde-led research develops world's highest gain high-power laser amplifier

The world's highest gain high power laser amplifier - by many orders of magnitude - has been developed in research led at the University of Strathclyde.

The researchers demonstrated the feasibility of using plasma to amplify short laser pulses of picojoule-level energy up to 100 millijoules, which is a 'gain'...

Im Focus: Can the immune system be boosted against Staphylococcus aureus by delivery of messenger RNA?

Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.

Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....

Im Focus: A quantum walk of photons

Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.

The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....

Im Focus: Turmoil in sluggish electrons’ existence

An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.

We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...

Im Focus: Wafer-thin Magnetic Materials Developed for Future Quantum Technologies

Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.

Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Marine Conservation: IASS Contributes to UN Ocean Conference in New York on 5-9 June

24.05.2017 | Event News

AWK Aachen Machine Tool Colloquium 2017: Internet of Production for Agile Enterprises

23.05.2017 | Event News

Dortmund MST Conference presents Individualized Healthcare Solutions with micro and nanotechnology

22.05.2017 | Event News

 
Latest News

Reptile vocalization is surprisingly flexible

30.05.2017 | Life Sciences

EU research project DEMETER strives for innovation in enzyme production technology

30.05.2017 | Power and Electrical Engineering

New insights into the ancestors of all complex life

29.05.2017 | Earth Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>