Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Go with the Flow

05.06.2014

Flow-through peptide synthesis and cell-based assays on Teflon-coated paper

The synthesis of peptides and proteins is an extremely complex matter, because they must be built up one amino acid at a time in repeated reaction and washing steps. Solid-phase synthesis has made this easier. Canadian researchers introduced a new variant of this technique in the journal Angewandte Chemie.


In their system, parallel syntheses take place in small regions on a paper support. The resulting peptide arrangements can be used in cell-based assays or in the search for 3D materials that support cell adhesion and growth.

In classical solid-phase synthesis, the growing peptide chain is fixed to a solid support—usually a polymer bead—so that the reagents can be rapidly and easily washed away after each step. Parallel solid-phase synthesis, known as SPOT synthesis, was developed as an alternative in the 1990s. This allows a large number of peptides to be obtained on a planar support with a small surface area.

SPOT synthesis has since been adapted for other applications, such as cell-based screening. The problem is that existing SPOT systems are not well-suited for chemical reactions. When individual drops of reagent are added by pipette, they wet small areas of the membrane—the SPOTs.

The circular spot of solvent absorbed by the membrane determines the size of the “reaction vessel”. Unlike in classical solid-phase synthesis, this limits the amounts of reagent, and flow-through conditions are not possible. This significantly limits the possible yields of the reactions.

A team headed by Frédérique Deiss and Ratmir Derda at the University of Alberta (Canada) has now found an elegant solution to this problem. The researchers used a Teflon coating to form a pattern of solvent-repellent barriers on a paper support. The pattern restricts the liquids to specific Teflon-free zones on the paper, forming small “reaction vessels” that can hold a larger volume than the usual SPOTs.

This not only allows for the use of excess volumes of reagents, but also allows for a flow-through reaction because the larger volume ensures for gravity-driven flow of the reagent solution through the paper. The flow rate can be varied by using paper of different porosity. This significantly improves yields.

There is an additional advantage to this method: the paper can be stacked or folded into thicker three-dimensional structures. The researchers were able to identify various peptides among those immobilized on the surface that support cell adhesion, growth, or differentiation in a three-dimensional environment.

About the Author

Ratmir Derda started his career as an assistant professor at the department of Chemistry at the University of Alberta in 2011. He is a principal investigator at the Alberta Glycomics Centre and Sentinel Bioactive Paper Network. In 2012, he received a Rising Star in Global Health Award from Grand Challenges Canada.

Author: Ratmir Derda, University of Alberta, Edmonton (Canada), http://derda.chem.ualberta.ca/contact/

Title: Flow-Through Synthesis on Teflon-Patterned Paper to Produce Peptide Arrays That Can Be Used for Cell-Based Assays

Angewandte Chemie International Edition, Permalink to the article: http://dx.doi.org/10.1002/anie.201402037

Ratmir Derda | Angewandte Chemie

Further reports about: Flow SPOT adhesion cell-based classical peptides reaction reactions synthesis volume

More articles from Life Sciences:

nachricht Toward a 'smart' patch that automatically delivers insulin when needed
18.01.2017 | American Chemical Society

nachricht 127 at one blow...
18.01.2017 | Stiftung Zoologisches Forschungsmuseum Alexander Koenig, Leibniz-Institut für Biodiversität der Tiere

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

Im Focus: Designing Architecture with Solar Building Envelopes

Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.

As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...

Im Focus: How to inflate a hardened concrete shell with a weight of 80 t

At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).

Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

Nothing will happen without batteries making it happen!

05.01.2017 | Event News

 
Latest News

A big nano boost for solar cells

18.01.2017 | Power and Electrical Engineering

Glass's off-kilter harmonies

18.01.2017 | Materials Sciences

Toward a 'smart' patch that automatically delivers insulin when needed

18.01.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>