Researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have unveiled the first glycosyltransferase clone collection specifically targeted for the study of the biosynthesis of plant cell walls.
The idea behind what is being called “the JBEI GT Collection” is to provide a functional genomic resource for researchers seeking to extract the sugars in plant biomass and synthesize them into clean, green and renewable transportation fuels.
Glycosyltransferases (GTs) are enzymes that catalyze the connection of simple monosaccharide sugars into the complex polysaccharide sugars that are essential to a wide range of plant cell structures and processes. While it is known that plants have evolved large families of GTs, the chemical nature of these enzymes is such that the specific functions of most GTs remain largely unknown. This is a major drawback for bioenergy research where the goal is to modify plant biomass for maximum fuel yields.
To address this problem, especially as it pertains to cell wall biosynthesis, a large team of JBEI researchers, led by Joshua Heazlewood, director of JBEI’s Plant Systems Biology program, has cloned and verified a clone library consisting of 403 Arabidopsis GTs and 96 rice GTs. In plant biology, Arabidopsis is the reference plant for species like poplar, and rice the reference plant for grasses.
“Using the unique infrastructure and resources at JBEI, we have provided a collection of high quality GT clones, all of which have been verified by sequencing and are available in easy to use cassettes,” Heazelwood says. “We’re making this entire collection available to the plant research community and expect it to drive our basic understanding of GTs and enable the manipulation of cell walls.”
In addition to the clones for Arabidopsis and rice GTs, Heazlewood and his collaborators at JBEI also created a set of highly efficient particle bombardment plasmids – pBullets – which are plasmids shot into a cell to mark the location of targeted proteins. The JBEI pBullets are constructed with markers for the plant endomembrane system, the collection of membranes that separates a cell’s functional and structural compartments.
“Our pBullet vector series is custom designed for efficient bombardment,” Heazlewood says. “Researchers generally use large unwieldy plasmids that perform badly when it comes to localizing proteins.”
While the 403 Arabidopsis clones represent approximately 88-percent of the defined Arabidopsis GTs, the 96 rice clones represent only 15-percent of the defined rice GTs. JBEI researchers are now working to expand this. Both the JBEI GT Collection and pBullet vector series are available to the research community through various outlets. For more information visit the Website at http://gt.jbei.org/
Heazlewood and his collaborators have published a paper on the JBEI GT Collection in The Plant Journal. The paper is titled “The Plant Glycosyltransferase Clone Collection for Functional Genomics.” Co-authors were Jeemeng Lao, Ai Oikawa, Jennifer Bromley, Peter McInerney, Anongpat Suttangkakul, Andreia Smith-Moritz, Hector Plahar, Tsan-Yu Chiu, Susana González Fernández-Niño, Berit Ebert, Fan Yang, Katy Christiansen, Sara Hansen, Solomon Stonebloom, Paul Adams, Pamela Ronald, Nathan Hillson, Masood Hadi, Miguel Vega-Sánchez, Dominique Loqué and Henrik Scheller.
This research was funded by the U.S. Department of Energy’s Office of Science.
Additional Information For more about the Joint BioEnergy Institute go here
Lynn Yarris | Eurek Alert!
Don't Give the Slightest Chance to Toxic Elements in Medicinal Products
23.03.2018 | Physikalisch-Technische Bundesanstalt (PTB)
North and South Cooperation to Combat Tuberculosis
22.03.2018 | Universität Zürich
Satellites in near-Earth orbit are at risk due to the steady increase in space debris. But their mission in the areas of telecommunications, navigation or weather forecasts is essential for society. Fraunhofer FHR therefore develops radar-based systems which allow the detection, tracking and cataloging of even the smallest particles of debris. Satellite operators who have access to our data are in a better position to plan evasive maneuvers and prevent destructive collisions. From April, 25-29 2018, Fraunhofer FHR and its partners will exhibit the complementary radar systems TIRA and GESTRA as well as the latest radar techniques for space observation across three stands at the ILA Berlin.
The "traffic situation" in space is very tense: the Earth is currently being orbited not only by countless satellites but also by a large volume of space...
An international team of researchers has discovered a new anti-cancer protein. The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. The researchers led by Prof. Michael N. Hall from the Biozentrum, University of Basel, report in “Nature” that LHPP can also serve as a biomarker for the diagnosis and prognosis of liver cancer.
The incidence of liver cancer, also known as hepatocellular carcinoma, is steadily increasing. In the last twenty years, the number of cases has almost doubled...
In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.
Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...
Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.
They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...
A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...
23.03.2018 | Event News
19.03.2018 | Event News
16.03.2018 | Event News
23.03.2018 | Life Sciences
23.03.2018 | Materials Sciences
23.03.2018 | Process Engineering