The research, conducted by members of Virginia Tech's Microelectromechanical Systems Laboratory (MEMS) Laboratory in the Bradley Department of Electrical and Computer Engineering, is the focus of a recent article in the Institute of Electrical and Electronic Engineers' Journal of Microelectomechanical Systems.
In their engineering laboratory, the researchers developed a new microfabrication technique to develop three-dimensional microfluidic devices in polymers. Microfluidics deals with the performance, control, and treatment of fluids that are constrained in some fashion, explained Masoud Agah , director of the laboratory.
As a result of this work, Agah, associate professor of the Bradley Department of Electrical and Computer Engineering and of the Virginia Tech–Wake Forest School of Biomedical Engineering and Sciences, and Amy Pruden, professor of civil and environmental engineering at Virginia Tech, have received a National Science Foundation award of $353,091 to use the technology and develop new microchips named 3D-ðDEP standing for "three-dimensional, passivated-electrode, insulator-based dielectrophoresis" for pathogen detection.
The NSF grant will allow them to focus on the isolation of waterborne pathogens that represent one of the "grand challenges to human health, costing the lives of about 2.5 million people worldwide each year," Agah and Pruden said.
According to the World Health Organization, the isolation of pathogenic bacteria from the environment has not significantly changed since the 1960s, when methods for chemical treatment of samples to remove background organisms were first implemented.
In the past, Agah said, researchers have mainly used two-dimensional microfluidic structures since this type of fabrication is more simplistic. With the three-dimensional device developed by Agah and his collaborators, Yayha Hosseini and Phillip Zellner, both graduate students in the department, they are able to customize the shapes of the channels and cavities of the devices the fluids passed through.
As an example, in human blood vessels, cells interact with each other and their surrounding environment inside circular channels. They have varying diameters, along with multiple branching and joints.
"Only under this type of condition can one truly study the biology of cells within a system in vitro as if it is occurring in vivo – our new microfluidic fabrication technology can resemble more realistically the structures of a cell's true living conditions," Agah said. It is the introduction of the three-dimensions that provides this distinctive environment.The combination of Agah and Pruden's expertise is important to the NSF-awarded work.
By blending their proficiencies, with Agah's group designing, modeling, and fabricating the chips, and Pruden's group preparing the different bacterial cultures for characterizing their dielectrophoresis properties and benchmarking it against more acceptable yet costly methods, they believe they will be able to isolate different pathogenic and nonpathogenic bacteria.
To make their three-dimensional structure, the Virginia Tech researchers used the material polydimethylsixolane, known for its elastic properties similar to rubber. This material is already widely used because of its transparency, biocompatibility, and low-cost.
"Our work establishes a reliable and robust, yet low-cost technique for the fabrication of versatile 3-D structures in polydimethylsixolane," Agad said.
Microfluidic devices can be used to trap and sort living organisms such as bacteria, viruses, and cells. With this new three-dimensional device that has a higher sensitivity and throughput than the two-dimensional version, according to Agah, he is able to make their predictions of applications ranging from water and food safety to fighting biological and chemical terrorism and to healthcare by fishing for abnormal cells in body fluids.
Both Hosseini of Kashan, Iran, and Zellner of Hampton, Va., are working on their doctoral degrees. Zellner is a SMART scholarship recipient from the Department of Defense.
Lynn Nystrom | EurekAlert!
Researchers pave the way for ionotronic nanodevices
23.02.2017 | Aalto University
Microhotplates for a smart gas sensor
22.02.2017 | Toyohashi University of Technology
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
24.02.2017 | Life Sciences
24.02.2017 | Life Sciences
24.02.2017 | Trade Fair News