Researchers from the INRA Plant Genomics Research Unit at Evry, and the INRA Grain Legumes Research Unit at Bretenières, both in France, developed a high-quality genetic reference collection of Pisum sativum mutants within the European Grain Legumes Integrated Project. Abdelhafid Bendahmane and colleagues used plants from an early-flowering garden pea cultivar, Caméor, to create a mutant population, which they then systematically phenotyped for use in both forward and reverse genetics studies.
The team set up a pea TILLING (Targeting Induced Local Lesions IN Genomes) platform with DNA samples from 4,704} plants. The TILLING technique overcomes the pea’s natural unsuitability to genetic modification techniques, and provides a powerful tool for investigating the role of essential genes. This new tool has implications for both basic science and for crop improvement. TILLING is an alternative to Agrobacterium-based techniques, and uses EMS (ethane methyl sulfonate) mutagenesis coupled with a gene-specific detection of single-nucleotide mutations. This reverse genetic strategy can be applied to all types of organisms and can be automated for high-throughput approaches.
Following this study, the researchers created a database called UTILLdb, which described each mutant plant at different developmental stages, (from seedling through to fruit maturation), and also incorporates digital images of the plants. UTILLdb contains phenotypic as well as sequence information on mutant genes, and can be searched for TILLING alleles of genes of interest, using the ‘BLAST’ tool, and for plant traits of interest, using keyword searches.
“By opening UTILLdb to the community, we hope to fulfil the expectations of both crop breeders and scientists who are using the pea as their model of study,” said research coordinator Abdelhafid Bendahmane.
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An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.
Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...
Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.
Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...
Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.
As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...
Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.
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Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine
Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...
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