Researchers from the INRA Plant Genomics Research Unit at Evry, and the INRA Grain Legumes Research Unit at Bretenières, both in France, developed a high-quality genetic reference collection of Pisum sativum mutants within the European Grain Legumes Integrated Project. Abdelhafid Bendahmane and colleagues used plants from an early-flowering garden pea cultivar, Caméor, to create a mutant population, which they then systematically phenotyped for use in both forward and reverse genetics studies.
The team set up a pea TILLING (Targeting Induced Local Lesions IN Genomes) platform with DNA samples from 4,704} plants. The TILLING technique overcomes the pea’s natural unsuitability to genetic modification techniques, and provides a powerful tool for investigating the role of essential genes. This new tool has implications for both basic science and for crop improvement. TILLING is an alternative to Agrobacterium-based techniques, and uses EMS (ethane methyl sulfonate) mutagenesis coupled with a gene-specific detection of single-nucleotide mutations. This reverse genetic strategy can be applied to all types of organisms and can be automated for high-throughput approaches.
Following this study, the researchers created a database called UTILLdb, which described each mutant plant at different developmental stages, (from seedling through to fruit maturation), and also incorporates digital images of the plants. UTILLdb contains phenotypic as well as sequence information on mutant genes, and can be searched for TILLING alleles of genes of interest, using the ‘BLAST’ tool, and for plant traits of interest, using keyword searches.
“By opening UTILLdb to the community, we hope to fulfil the expectations of both crop breeders and scientists who are using the pea as their model of study,” said research coordinator Abdelhafid Bendahmane.
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More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.
Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...
Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.
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The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
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