Simulating evolution to understand a hidden switch

Using computer simulations built on reasonable assumptions and conducted under careful control, computational bioscientists can mimic real biological conditions. Starting with the original founding population (ancient phase), they can evolve the population over several thousand generations to develop an intermediate phase, and then evolve that generation another several thousand generations to develop a derived phase.
Credit: © 2021 KAUST; Anastasia Serin

Computer simulations of cells evolving over tens of thousands of generations reveal why some organisms retain a disused switch mechanism that turns on under severe stress, changing some of their characteristics. Maintaining this “hidden” switch is one means for organisms to maintain a high degree of gene expression stability under normal conditions.

Tomato hornworm larvae are green in warmer regions, making camouflage easier, but black in cooler temperatures so that they can absorb more sunlight. This phenomenon, found in some organisms, is called phenotypic switching. Normally hidden, this switching is activated in response to dangerous genetic or environmental changes.

Scientists have typically studied this process by investigating the changes undergone by organisms under different circumstances over many generations. Several years ago, for example, a team bred generations of tobacco hornworm larvae to observe and induce color changes similar to those that occurred in their tomato hornworm relatives.

“Computer simulations, when built on reasonable assumptions and conducted under careful control, are a very powerful tool to mimic the real situation,” says KAUST computational bioscientist Xin Gao. “This helps scientists observe and understand principles that are otherwise very difficult, or impossible, to observe by wet-lab experiments.”

Gao and KAUST research scientist Hiroyuki Kuwahara designed a computer simulation of the evolution of 1,000 asexual microorganisms. Each organism was given a gene circuit model for regulating the expression of a specific protein X.

The simulation evolved the population over 90,000 generations. The original founding population had identical nonswitching gene circuits and evolved over 30,000 generations, collectively called the ancient population, under stable conditions. The next 30,000 generations, called the intermediate population, were exposed to fluctuating environments that switched every 20 generations. The final 30,000 generations, the derived population, were exposed to a stable environment.

The individuals in the ancient and derived populations, who evolved in stable environments, both had gene expression levels that were optimized for stability. But they were different: the ancient population’s stability did not involve phenotypic switching, while the derived population’s did. The difference, explains Kuwahara, stems from the intermediate population, in which switching was favored in order to deal with the fluctuating conditions.

The simulations suggest that populations of organisms maintain their switching machinery over a long period of environmental stability by gradually evolving low-threshold switches, which easily switch in fluctuating circumstances, to high-threshold switches when the environment is more stable.

This is easier, says Kuwahara, than reverting to a nonswitching state through small mutational shifts. “Instead, we end up with a type of ‘hidden’ phenotypic switching that acts like an evolutionary capacitor, storing genetic variations and releasing alternative phenotypes in the event of substantial perturbations,” Kuwahara says.

The team next plans to use computer simulations to study more complex biological systems while also interactively collaborating with researchers conducting wet-lab experiments. Their aim is to develop theoretical frameworks that can be experimentally validated.

Media Contact

Michael Cusack
King Abdullah University of Science & Technology (KAUST)

All latest news from the category: Information Technology

Here you can find a summary of innovations in the fields of information and data processing and up-to-date developments on IT equipment and hardware.

This area covers topics such as IT services, IT architectures, IT management and telecommunications.

Back to home

Comments (0)

Write a comment

Newest articles

New species of microalgae discovered

Ultrasmall microalgae found in home aquarium could have multiple useful applications. A new species of microalgae was found in water from a home aquarium. While analyzing DNA samples taken from…

New technology may help inform brain stimulation

Brain stimulation, such as Deep brain stimulation (DBS), is a powerful way to treat neurological and psychiatric disorders. While it has provided therapeutic benefit for sufferers of Parkinson’s, Alzheimer’s, and…

New hybrid catalyst could help decarbonization

… and make ethylene production more sustainable. A new hybrid catalyst converts carbon dioxide into ethylene in one pot. The catalyst was developed by scientists from Ames National Laboratory, Iowa…

Partners & Sponsors