The invention at hand provides a novel protein for the chemoenzymatic and enantiomerically pure production of L-<i>threo</i>-hydroxyaspartate from L-aspartate.<p> The naturally occurring protein AsnO (asparagine oxygenase) is part of the CDA biosynthesis gene cluster in Streptomyces coelicor. AsnO is an Fe<sup>2+</sup> and <font face="symbol">a</font>-ketoglutarate-dependent hydroxylase. This hydroxylase acts as a building block for non-ribosomally produced CDA (?Calcium-dependent antibiotic?) in vivo. During the catalytic cycle, this class of enzymes couples the oxidative decomposition of <font face="symbol">a</font>-ketoglutarate to succinate and CO<sub>2</sub> with the hydroxylation of the substrate (L-asparagine).<p> In the wild type of AsnO, the side chain of the amino acid residue Asp-241 thereby binds to the NH<sub>2</sub> group of the carboxamide group of L-Asn. Surprisingly, it was found that directed mutagenesis of Asp-241 to Asn-241 (D241N) results in a binding site for the carboxyl group of an aspartate side chain. By means of this directed mutagenesis, the substrate specificity changes from asparagine to aspartate, and the mutated protein converts aspartate chemoenzymatically to L-threo-hydroxyaspartate in the presence of Fe2+ and a-ketoglutarate.<p> Thereby, AsnO D241N represents the mutant of the wild type AsnO according to the present invention.
Further information: PDF
TransMIT Gesellschaft für Technologietransfer mbH
Phone: +49 (0)641/943 64-12
Dr. Peter Stumpf
As Germany's association of technology- and patenttransfer agencies TechnologieAllianz e.V. is offering businesses access to the entire range of innovative research results of almost all German universities and numerous non-university research institutions. More than 2000 technology offers of 14 branches are beeing made accessable to businesses in order to assure your advance on the market. At www.technologieallianz.de a free, fast and non-bureaucratic access to all further offers of the German research landscape is offered to our members aiming to sucessfully transfer technologies.
email@example.com | TechnologieAllianz e.V.
New Lithium Salts of Pentafluorophenylamide Anions as Electrolytes in Lithium Ionic Batteries
18.04.2017 | TechnologieAllianz e.V.
Gratings on glass surfaces
28.03.2017 | TechnologieAllianz e.V.
A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.
The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
20.07.2018 | Power and Electrical Engineering
20.07.2018 | Information Technology
20.07.2018 | Materials Sciences