One-Plasmid Systems for Blue-light-regulated Gene Expression in Prokaryotes
<strong>Background</strong><br>
Protein expression systems are very widely used in the life sciences, biotechnology and medicine. Traditional strategies for recombinant protein expression involve the transformation of a gene expression vector into prokaryotic expression hosts and culturing the cells so that they express the desired protein. Using an inducible expression cassette the onset of the protein expression can be controlled by several inducer (f.e. chemical, temperature, cold).<br><br><strong>Technology</strong><br> We offer two compact plasmids for facile light regulated expression of target proteins in prokaryotic hosts. Dependent from the application, one plasmid can be used for light activated and one for light inactivated gene expression. Both plasmids are working without exogenous addition of chromophores or expensive chemical inducers and allow a specific, spatially- and temporally precise control of protein expression. A competitive advantage is the use of light as inducer especially for the production of recombinant proteins. Another advantage is the very precise control of protein expression which is very relevant for several research applications. By using standard chemical inducers a spatially and temporally precise control of protein expression is not possible.<br> <strong><br><br> <b>Benefits:</b> <ul> <li>Both plasmids are compact and ready-to-use</li> <li>No exogenous addition of chromophores or chemical inducers is required and thus reduced production costs</li> <li>System facilitates pulsed and/or automated induction of protein expression</li> <li>Specific, spatially- and temporally-precise protein expression</li> </ul><br><br> <p><strong>IP Rights</strong><br> European Patent Applic. (06/2009).<br> PCT Application (08/2010). <br><br> <strong>Patent Owner</strong><br> Humboldt-Universität zu Berlin <b>Find the technology offer and contact details here: <a href=”http://www.ipal.de/technologieangebote/11043″ target=”_blank”>http://www.ipal.de/technologieangebote/11043</a></b>
Further Information: PDF
ipal GmbH
Phone: +49 (0)30/2125-4820
Contact
Dr. Dirk Dantz
Media Contact
All latest news from the category: Technology Offerings
Newest articles
Microscopic basis of a new form of quantum magnetism
Not all magnets are the same. When we think of magnetism, we often think of magnets that stick to a refrigerator’s door. For these types of magnets, the electronic interactions…
An epigenome editing toolkit to dissect the mechanisms of gene regulation
A study from the Hackett group at EMBL Rome led to the development of a powerful epigenetic editing technology, which unlocks the ability to precisely program chromatin modifications. Understanding how…
NASA selects UF mission to better track the Earth’s water and ice
NASA has selected a team of University of Florida aerospace engineers to pursue a groundbreaking $12 million mission aimed at improving the way we track changes in Earth’s structures, such…
