Patients with a rare disease, called narcolepsy, suffer of excessive daytime sleepiness and cataplexy (brief episodes of loss of muscle tone triggered by emotions). A study published in the renowned scientific journal Nature reports, for the first time, the existence in patients with narcolepsy of autoreactive T lymphocytes (cells of the immune system) that recognize hypocretin and can mediate an immune response leading to loss of hypocretin-producing neurons. This study identifies the culprit of this enigmatic disease and has major implications for its diagnosis and therapy.
Patients with a rare disease, called narcolepsy, suffer of excessive daytime sleepiness and cataplexy (brief episodes of loss of muscle tone triggered by emotions). The disease, first described in 1877, depends on genetic and environmental factors and is caused by the loss in the brain of some neurons that produce a neurotransmitter called hypocretin, but the underlying mechanism remained mysterious.
A study published in the renowned scientific journal Nature reports, for the first time, the existence in patients with narcolepsy of autoreactive T lymphocytes (cells of the immune system) that recognize hypocretin and can mediate an immune response leading to loss of hypocretin-producing neurons. This study identifies the culprit of this enigmatic disease and has major implications for its diagnosis and therapy.
The study is the result of a close collaboration between basic and clinical scientists and was jointly coordinated by Prof. Federica Sallusto at the Institute for Research in Biomedicine in Bellinzona (IRB, affiliated to USI Università della Svizzera italiana) and at ETH Zurich, and Prof. Claudio Bassetti at the University Sleep-Wake-Epilepsy-Center Bern of the Department of Neurology at the University Hospital (Inselspital) in Bern.
The study also involved the Neurocenter of Southern Switzerland of the Ente Ospedaliero Cantonale, the Center for Sleep Research and Sleep Medicine of the Clinic Barmelweid, the Department of Physiology at the University of Lausanne, the Institute of Experimental Immunology at the University of Zurich, and the Institute of Immunology at the University Witten/Herdecke in Germany. The work was supported by the Swiss National Science Foundation, the European Research Council (ERC) and the Helmut Horten Foundation.
According to Prof. Federica Sallusto: «Using new sensitive methods we have been able to identify T lymphocytes reactive against hypocretin as the culprit of this disease. These autoreactive T lymphocytes can cause inflammation leading to neuronal damage or even kill hypocretin-producing neurons. By blocking them at early stages, it may be possible to limit neuronal loss and prevent progression of the disease.»
According to Prof. Claudio L.A. Bassetti: «This publication will increase the awareness about narcolepsy, which remains poorly known in the general population and often is not, or belatedly, diagnosed by physicians. This study will also open new opportunities for an early diagnosis and novel treatment approaches of this disabling disease.»
Narcolepsy is a rare chronic brain disorder, which affects about 0.05% of the general population and manifests with excessive daytime drowsiness (with “sleep attacks”), cataplexy (loss of muscle control, typically triggered by a sudden, positive emotion), sleep paralysis, hallucinations, and disturbed sleep. The cause of narcolepsy is the loss of neurons in the hypothalamus (a region of the brain), which produces hypocretin (HCRT), a protein which regulates sleep-wake, emotional and feeding behaviors. The presence in over 95% of patients of a specific genetic marker (the HLA allele DQB1*0602) suggests that narcolepsy could be an autoimmune disease. The observations of identical twins that are discordant for the disease (one has narcolepsy, the other one not) and of an increased frequency of narcolepsy after certain infections or types of influenza vaccinations suggest the potential role of environmental factors as triggers of the autoimmune process. However, the mechanisms involved in the loss of hypocretin neurons remained until today unknown.
In this study, the IRB researchers used a sensitive method that they have developed in Bellinzona to interrogate the T lymphocyte repertoire of narcolepsy patients to identify, for the first time, T lymphocytes of the CD4 and, in some cases, of the CD8 type that react against hypocretin and against other proteins expressed in hypocretin neurons. These autoreactive lymphocytes can cause inflammation leading to neuronal damage or even kill hypocretin-producing neurons. They also identified the molecular interactions leading to hypocretin recognition and a possible mechanism by which the autoreactive T cells may have escaped tolerance. Finally, in the patients studied, it was possible to exclude a role for the influenza virus in the induction of T lymphocytes reactive against hypocretin.
About the Institute for Research in Biomedicine (IRB)
The Institute for Research in Biomedicine (IRB), founded in 2000 in Bellinzona, was affiliated to Università della Svizzera italiana (USI) in 2010. Supported by private and public institutions and by competitive research grants, the IRB currently hosts thirteen research groups and 120 researchers. Research at the IRB focuses on the human host defense against infections, tumors and degenerative diseases. With more than 580 publications in leading scientific journals, the IRB has gained an international reputation as a center of excellence in immunology. http://www.irb.usi.ch
About the Department of Neurology, at Inselspital, Bern University Hospital
The Department of Neurology at Inselspital, Bern University Hospital, with over 140 academics and more than 600 employees, is the leading academic neurological center in Switzerland. Its international reputation is based on excellence of clinical care, teaching and research in stroke, sleep/epilepsy, Parkinsonism/movement disorders, neuroimmunology, and neurorehabilitation. In the last few months, the University of Bern provided CHF 6 million for the Interfaculty Research Cooperation project «Decoding sleep: from neurons to health & mind» (PI: Claudio Bassetti). In addition, two ERC consolidator grants worth a total of CHF 4 million started in the department (PI’s: Antoine Adamantidis and Smita Saxena). http://www.neurologie.insel.ch
The arcticle «T cells in patients with narcolepsy target self-antigens of hypocretin neurons» (authors: Daniela Latorre, Ulf Kallweit, Eric Armentani, Mathilde Foglierini, Federico Mele, Antonino Cassotta, Sandra Jovic, David Jarrossay, Johannes Mathis, Francesco Zellini, Burkhard Becher, Antonio Lanzavecchia, Ramin Khatami, Mauro Manconi, Mehdi Tafti, Claudio L. Bassetti, Federica Sallusto) is available after the indicated embargo time at http://www.nature.com.
For questions on basic research:
Prof. Federica Sallusto, Institute for Research in Biomedicine (IRB)
+41 91 820 0315
For questions about clinical research:
Prof. Dr. med. Claudio L.A. Bassetti, Department of Neurology, Inselspital, Bern University Hospital
+41 31 632 30 66
Stephanie Falk | idw - Informationsdienst Wissenschaft
Photoactive bacteria bait may help in fight against MRSA infections
12.10.2018 | Purdue University
15 emerging technologies that could reduce global catastrophic biological risks
10.10.2018 | Johns Hopkins Center for Health Security
Scientists at the Max Planck Institute for Polymer Research (MPI-P) in Mainz (Germany) together with scientists from Dresden, Leipzig, Sofia (Bulgaria) and Madrid (Spain) have now developed and characterized a novel, metal-organic material which displays electrical properties mimicking those of highly crystalline silicon. The material which can easily be fabricated at room temperature could serve as a replacement for expensive conventional inorganic materials used in optoelectronics.
Silicon, a so called semiconductor, is currently widely employed for the development of components such as solar cells, LEDs or computer chips. High purity...
Augsburg chemists present a new technology for compressing, storing and transporting highly volatile gases in porous frameworks/New prospects for gas-powered vehicles
Storage of highly volatile gases has always been a major technological challenge, not least for use in the automotive sector, for, for example, methane or...
When we put water in a freezer, water molecules crystallize and form ice. This change from one phase of matter to another is called a phase transition. While this transition, and countless others that occur in nature, typically takes place at the same fixed conditions, such as the freezing point, one can ask how it can be influenced in a controlled way.
We are all familiar with such control of the freezing transition, as it is an essential ingredient in the art of making a sorbet or a slushy. To make a cold...
Thin organic layers provide machines and equipment with new functions. They enable, for example, tiny energy recuperators. In future, these will be installed...
Das Zusammenspiel aus Struktur und Dynamik bestimmt die Funktion von Proteinen, den molekularen Werkzeugen der Zelle. Durch Fortschritte in der...
17.10.2018 | Event News
16.10.2018 | Event News
02.10.2018 | Event News
18.10.2018 | Life Sciences
17.10.2018 | Trade Fair News
17.10.2018 | Life Sciences