These scientific advances, achieved by Professor Michel Sylvestre of Centre INRS–Institut Armand-Frappier in conjunction with U.S. and Indian researchers, will serve not only to help develop effective biocatalysts for resolving environmental pollution problems, but also to synthesize new chemical compounds of biopharmaceutical interest.
Certain chemical components, like PCBs, PAHs, and CFCs, are toxic biosphere pollutants that are resistant to microbial degradation. Microbial catabolic enzymes are unable to effectively metabolize them. The results obtained by Professor Sylvestre and his colleagues open up new possibilities for boosting the effectiveness of these enzymes to oxidize such compounds.
Professor Sylvestre's research team has shown that it is possible to obtain more flexible mutant enzymes by replacing some of their amino acids. Moreover, they have updated a sophisticated mechanism that helps boost the enzyme's performance not only with regard to the natural substrate, but also any other substrates it can metabolize. As such, more effective new enzymes can be developed using genetic engineering.
"From a green chemistry perspective, the results of our research could allow us to apply these enzymes to biocatalysis processes to synthesize biologically active compounds (such as flavonoids) that have strong antioxidant properties," explained Professor Michel Sylvestre, also an enzyme engineering specialist.
The results were published in the following works: Mohammadi, M., Viger, J.F., Kumar, P., Barriault, D., Bolin, J. T., Sylvestre, M. 2011. "Retuning Rieske-type oxygenase to expand substrate range." J. Biol Chem. 286, 27612-27621. http://www.jbc.org/content/286/31/27612.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095
Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, L.D., Bolin, J. T., and Sylvestre, M. 2011. "Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution." J. Mol. Biol. 405, 531-547. http://www.sciencedirect.com/science/article/pii/S002228361001209X
Dhindwal, S., D. N. Patil, M. Mohammadi, M. Sylvestre, S. Tomar, and P. Kumar. 2011. "Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme." J. Biol. Chem. 286, 37011-37022. http://www.jbc.org/content/286/42/37011.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095
Institut national de recherche scientifique (INRS) is a graduate and postgraduate research and training university. One of Canada's leading research universities in terms of grants per professor, INRS brings together some 150 professors and close to 700 students and postdoctoral fellows in its centres in Montreal, Quebec City, Laval, and Varennes. INRS research teams conduct fundamental research essential to the advancement of science in Quebec as well as internationally and play a critical role in developing concrete solutions to problems facing our society.
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