These scientific advances, achieved by Professor Michel Sylvestre of Centre INRS–Institut Armand-Frappier in conjunction with U.S. and Indian researchers, will serve not only to help develop effective biocatalysts for resolving environmental pollution problems, but also to synthesize new chemical compounds of biopharmaceutical interest.
Certain chemical components, like PCBs, PAHs, and CFCs, are toxic biosphere pollutants that are resistant to microbial degradation. Microbial catabolic enzymes are unable to effectively metabolize them. The results obtained by Professor Sylvestre and his colleagues open up new possibilities for boosting the effectiveness of these enzymes to oxidize such compounds.
Professor Sylvestre's research team has shown that it is possible to obtain more flexible mutant enzymes by replacing some of their amino acids. Moreover, they have updated a sophisticated mechanism that helps boost the enzyme's performance not only with regard to the natural substrate, but also any other substrates it can metabolize. As such, more effective new enzymes can be developed using genetic engineering.
"From a green chemistry perspective, the results of our research could allow us to apply these enzymes to biocatalysis processes to synthesize biologically active compounds (such as flavonoids) that have strong antioxidant properties," explained Professor Michel Sylvestre, also an enzyme engineering specialist.
The results were published in the following works: Mohammadi, M., Viger, J.F., Kumar, P., Barriault, D., Bolin, J. T., Sylvestre, M. 2011. "Retuning Rieske-type oxygenase to expand substrate range." J. Biol Chem. 286, 27612-27621. http://www.jbc.org/content/286/31/27612.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095
Kumar, P., Mohammadi, M., Viger, J.F., Barriault, D., Gomez-Gil, L., Eltis, L.D., Bolin, J. T., and Sylvestre, M. 2011. "Structural insight into the expanded PCB-degrading abilities of a biphenyl dioxygenase obtained by directed evolution." J. Mol. Biol. 405, 531-547. http://www.sciencedirect.com/science/article/pii/S002228361001209X
Dhindwal, S., D. N. Patil, M. Mohammadi, M. Sylvestre, S. Tomar, and P. Kumar. 2011. "Biochemical studies and ligand bound structures of biphenyl dehydrogenase from Pandoraea pnomenusa strain B-356 reveal a basis for broad specificity of the enzyme." J. Biol. Chem. 286, 37011-37022. http://www.jbc.org/content/286/42/37011.abstract?sid=9a7395c8-4e4c-460a-a69a-932c85d04095
Institut national de recherche scientifique (INRS) is a graduate and postgraduate research and training university. One of Canada's leading research universities in terms of grants per professor, INRS brings together some 150 professors and close to 700 students and postdoctoral fellows in its centres in Montreal, Quebec City, Laval, and Varennes. INRS research teams conduct fundamental research essential to the advancement of science in Quebec as well as internationally and play a critical role in developing concrete solutions to problems facing our society.
Gisele Bolduc | EurekAlert!
New bioinformatics platform for the genome-based taxonomical classification of bacteria and archaea
21.05.2019 | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
3D technology lets us look into the distant past
21.05.2019 | Eberhard Karls Universität Tübingen
Engineers at the University of Tokyo continually pioneer new ways to improve battery technology. Professor Atsuo Yamada and his team recently developed a...
With a quantum coprocessor in the cloud, physicists from Innsbruck, Austria, open the door to the simulation of previously unsolvable problems in chemistry, materials research or high-energy physics. The research groups led by Rainer Blatt and Peter Zoller report in the journal Nature how they simulated particle physics phenomena on 20 quantum bits and how the quantum simulator self-verified the result for the first time.
Many scientists are currently working on investigating how quantum advantage can be exploited on hardware already available today. Three years ago, physicists...
'Quantum technologies' utilise the unique phenomena of quantum superposition and entanglement to encode and process information, with potentially profound benefits to a wide range of information technologies from communications to sensing and computing.
However a major challenge in developing these technologies is that the quantum phenomena are very fragile, and only a handful of physical systems have been...
Working group led by physicist Professor Ulrich Nowak at the University of Konstanz, in collaboration with a team of physicists from Johannes Gutenberg University Mainz, demonstrates how skyrmions can be used for the computer concepts of the future
When it comes to performing a calculation destined to arrive at an exact result, humans are hopelessly inferior to the computer. In other areas, humans are...
Scientists develop a molecular recording tool that enables in vivo lineage tracing of embryonic cells
The beginning of new life starts with a fascinating process: A single cell gives rise to progenitor cells that eventually differentiate into the three germ...
29.04.2019 | Event News
17.04.2019 | Event News
15.04.2019 | Event News
21.05.2019 | Physics and Astronomy
21.05.2019 | Earth Sciences
21.05.2019 | Life Sciences