The advance could improve a range of genetic diagnostics and screenings where precise measurement is crucial--including the early detection of cancer, prenatal diagnostics, the detection of pathogens in food products, and the analysis of single cell gene expression.
The new digital polymerase chain reaction (PCR) device uses liquid surface tension, rather than systems of microscopic valves, to partition DNA samples into arrays of 1,000,000 chambers or more. The device enables the direct counting of single molecules isolated in individual chambers.
The density of reaction chambers achieved by the platform exceeds more traditional valve-based digital PCR techniques by a factor of 100, translating directly into improved performance.
"This solves some major technical issues that have limited the scale and accuracy of traditional digital PCR techniques," says Assistant Professor Carl Hansen with the UBC Department of Physics and Astronomy and Centre for High]Throughput Biology. "It creates defect-free arrays of millions of uniform volume sub-reactions, and controls dehydration of these reactions during thermocycling."
PCR is an indispensable molecular biology technique used by researchers to amplify--or copy--a single piece of DNA millions or billions of times. The technique relies on repeated cycles of heating and cooling of the reaction to replicate segments of DNA using a protein called DNA polymerase, the same enzyme that copies DNA in living cells. PCR is used in medical and biology labs to clone DNA, analyze genes, detect hereditary disease, and in forensics.
The description of the 'megapixel' platform was published today in Nature Methods.
Digital PCR refers to a new generation of DNA replication techniques that offer increased sensitively and density over the original technique, developed in 1983. The greatest number of chambers available in commercially available implementations of digital PCR, using integrated micro-valves, is 36,960. However, further scalability is limited by the maximum density at which valves may be reliably fabricated.
Hansen believes the new version or digital PCR can be scaled to hold up to approximately 10,000,000 chambers on a standard one inch format.
The UBC researchers also found the new 'megapixel' technique set new benchmarks in detecting rare mutations--defined as the lowest measurable ratio of two target sequences differing by a single nucleotide variation as well as new limits in the detection of subtle differences in sequence abundance.
Partitioning of a one million chamber array takes approximately one minute.
"Our solution, or something using the same techniques, could enable a new degree of precision in measurements in biomedical research and diagnostics. The dramatic increase in assay density has important implications for the adoption of digital PCR as an economical, fast and routine analytical tool," says Hansen.
This research was funded by the Canadian Institute for Health Research, the Terry Fox Foundation, and the Natural Science and Engineering Research Council.
Megapixel Digital PCR http://dx.doi.org/10.1038/nmeth.1640
Nature Methods www.nature.com/nmeth
Carl Hansen | EurekAlert!
Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View
22.06.2018 | University of Sussex
New cellular pathway helps explain how inflammation leads to artery disease
22.06.2018 | Cedars-Sinai Medical Center
In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.
Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...
Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...
Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.
Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...
The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.
Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.
An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.
Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...
13.06.2018 | Event News
08.06.2018 | Event News
05.06.2018 | Event News
22.06.2018 | Materials Sciences
22.06.2018 | Earth Sciences
22.06.2018 | Life Sciences