Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Signaling Pathways to the Nucleus

19.03.2018

Researchers have demonstrated how auxin, a hormone that controls many processes in plants, reaches its destination

A team of researchers from the University of Freiburg have discovered how the plant hormone auxin is transported within the cell and how this signaling pathway helps to control gene expression in the nucleus. Auxin regulates many processes in plants: from embryonic development, to the development of organs, all the way to responses to changes in the environment. The team recently published its research in the journal Cell Reports.


The hormone auxin controls many processes in plants.

Source: Institute of Biology II/University of Freiburg

According to current scientific models, auxin works with other proteins to fulfill its function. When auxin content in the nucleus rises, receptors bind in the presence of auxin repressors, initiate repressor degradation and enable auxin responsive transcription factors to trigger gene expression. Because it is believed that auxin content in the nucleus is important for this, the researchers focused on how auxin gets into the nucleus and how this process is controlled.

The researchers from the University of Freiburg therefore collaborated with colleagues from Munich and Okayama, Japan, to test new, fluorescent, auxin-mimicking molecules in single cells. These molecules allowed them to visualize the accumulation of auxin in the cell without triggering any auxin-related processes. They were thus able to demonstrate that the auxin-mimicking molecules accumulated primarily in the endoplasmic reticulum (ER), which is a system of flat tubules that is a continuation of the nuclear membrane directly connected to the nucleus.

Next, the team studied how the ER, nucleus, and other cell parts work together to absorb auxin in the nucleus. Because there are currently no adequate methods of directly measuring the transport of auxin between the cell’s different compartments, the researchers developed a combined experimental-theoretical approach that allows them to use a combination of microscopy, quantitative data analysis, and mathematical modeling to observe how individual plant cells react to different auxin levels. Based on the results of their research, they concluded that the flow of auxin from the ER to the nucleus represents an important signaling pathway within the cell to regulate auxin levels in the nucleus, and hence in supporting processes triggered by auxin.

The team of researchers includes Dr. Cristina Dal Bosco, Dr. Alexander Dovzhenko, and Prof. Dr. Klaus Palme, all from the Department of Molecular Plant Physiology of the Institute of Biology II, as well as Dr. Alistair Middleton and Prof. Dr. Christian Fleck, both from the Center for Biological Systems Analysis (ZBSA) at the University of Freiburg. Dr. Palme is also a member of the cluster of excellence BIOSS Centre for Biological Signalling Studies.

Original Publication:
Middleton, A. M., Dal Bosco, C, Chlap, P, Bensch, R., Harz, H., Ren, F., Bergmann, S., Wend, S., Weber, W., Hayashi, K., Zurbriggen, M.D., Uhl, R., Ronneberger, O., Palme, K., Fleck, C., Dovzhenko, A. (2018): Data-driven modeling of intracellular auxin fluxes indicates a dominant role of the ER in controlling nuclear auxin uptake. Cell Reports.
www.cell.com/cell-reports/fulltext/S2211-1247(18)30269-9

Contact:
Prof. Dr. Klaus Palme
Institute of Biology II
University of Freiburg
Phone: +49 (0)761/203 - 2954
E-Mail: klaus.palme@biologie.uni-freiburg.de

Caption:
The hormone auxin controls many processes in plants. Source: Institute of Biology II/University of Freiburg

Weitere Informationen:

https://www.pr.uni-freiburg.de/pm-en/press-releases-2018/signaling-pathways-to-t...

Rudolf-Werner Dreier | Albert-Ludwigs-Universität Freiburg im Breisgau

More articles from Life Sciences:

nachricht Molecular motors run in unison in a metal-organic framework
20.03.2019 | University of Groningen

nachricht Active substance from plant slows down aggressive eye cancer
20.03.2019 | Rheinische Friedrich-Wilhelms-Universität Bonn

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Self-healing coating made of corn starch makes small scratches disappear through heat

Due to the special arrangement of its molecules, a new coating made of corn starch is able to repair small scratches by itself through heat: The cross-linking via ring-shaped molecules makes the material mobile, so that it compensates for the scratches and these disappear again.

Superficial micro-scratches on the car body or on other high-gloss surfaces are harmless, but annoying. Especially in the luxury segment such surfaces are...

Im Focus: Stellar cartography

The Potsdam Echelle Polarimetric and Spectroscopic Instrument (PEPSI) at the Large Binocular Telescope (LBT) in Arizona released its first image of the surface magnetic field of another star. In a paper in the European journal Astronomy & Astrophysics, the PEPSI team presents a Zeeman- Doppler-Image of the surface of the magnetically active star II Pegasi.

A special technique allows astronomers to resolve the surfaces of faraway stars. Those are otherwise only seen as point sources, even in the largest telescopes...

Im Focus: Heading towards a tsunami of light

Researchers at Chalmers University of Technology and the University of Gothenburg, Sweden, have proposed a way to create a completely new source of radiation. Ultra-intense light pulses consist of the motion of a single wave and can be described as a tsunami of light. The strong wave can be used to study interactions between matter and light in a unique way. Their research is now published in the scientific journal Physical Review Letters.

"This source of radiation lets us look at reality through a new angle - it is like twisting a mirror and discovering something completely different," says...

Im Focus: Revealing the secret of the vacuum for the first time

New research group at the University of Jena combines theory and experiment to demonstrate for the first time certain physical processes in a quantum vacuum

For most people, a vacuum is an empty space. Quantum physics, on the other hand, assumes that even in this lowest-energy state, particles and antiparticles...

Im Focus: Sussex scientists one step closer to a clock that could replace GPS and Galileo

Physicists in the EPic Lab at University of Sussex make crucial development in global race to develop a portable atomic clock

Scientists in the Emergent Photonics Lab (EPic Lab) at the University of Sussex have made a breakthrough to a crucial element of an atomic clock - devices...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

International Modelica Conference with 330 visitors from 21 countries at OTH Regensburg

11.03.2019 | Event News

Selection Completed: 580 Young Scientists from 88 Countries at the Lindau Nobel Laureate Meeting

01.03.2019 | Event News

LightMAT 2019 – 3rd International Conference on Light Materials – Science and Technology

28.02.2019 | Event News

 
Latest News

Molecular motors run in unison in a metal-organic framework

20.03.2019 | Life Sciences

Active substance from plant slows down aggressive eye cancer

20.03.2019 | Life Sciences

Novel sensor system improves reliability of high-temperature humidity measurements

20.03.2019 | Trade Fair News

VideoLinks
Science & Research
Overview of more VideoLinks >>>