Individual cells can pass resistance genes to one another through a process called horizontal gene transfer, or by “transformation,” the uptake of DNA from the environment.
Now researchers report that they can interrupt the cascade of cellular events that allows S. pneumoniae to swap or suck up DNA. The new findings, reported in the journal PLoS ONE, advance the effort to develop a reliable method for shutting down the spread of drug resistance in bacteria.
“Within the last few decades, S. pneumoniae has developed resistance to several classes of antibiotics,” said University of Illinois pathobiology professor Gee Lau, who led the study. “Importantly, it has been shown that antibiotic stress – the use of antibiotics to treat an infection – can actually induce the transfer of resistance genes among S. pneumoniae. Our approach inhibits resistance gene transfer in all strains of S. pneumoniae, and does so without increasing selective pressure and without increasing the likelihood that resistant strains will become dominant.”
Lau and his colleagues focused on blocking a protein that, when it binds to a receptor in the bacterial cell membrane, spurs a series of events in the cell that makes the bacterium “competent” to receive new genetic material. The researchers hypothesized that interfering with this protein (called CSP) would hinder its ability to promote gene transfer.
In previous work published late last year in the journal PLoS Pathogens, Lau’s team identified proteins that could be made in the lab that were structurally very similar to the CSP proteins. These artificial CSPs can dock with the membrane receptors, block the bacterial CSPs’ access to the receptors and reduce bacterial competence, as well as reducing the infectious capacity of S. pneumoniae.
In the new study, the researchers fine-tuned the amino acid structure of more than a dozen artificial CSPs and tested how well they inhibited the S. pneumoniae CSPs. They also tested their ability (or, more desirably, their inability) to mimic the activity of CSPs in bacterial cells.
“The chemical properties of individual amino acids in a protein can greatly influence the protein’s activity,” Lau said.
The team identified several artificial CSPs that both inhibited the bacterial CSPs and reduced S. pneumoniae competence by more than 90 percent.
“This strategy will likely help us reduce the spread of antibiotic-resistance genes among S. pneumoniae and perhaps other species of streptococcus bacteria,” Lau said.
The study team included researchers from Sun-Yat-Sen University in Guangdong, China. The National Institutes of Health and the University of Illinois Research Board Arnold O. Beckman Research Endowment partially supported this work.Editor’s notes: To reach Gee Lau, call 217-333-5077; email firstname.lastname@example.org.
Diana Yates | University of Illinois
Microscope measures muscle weakness
16.11.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg
Good preparation is half the digestion
16.11.2018 | Max-Planck-Institut für Stoffwechselforschung
Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.
Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
16.11.2018 | Health and Medicine
16.11.2018 | Life Sciences
16.11.2018 | Life Sciences