The movement and growth of cells are critical for normal physiological processes, and--when perturbed--can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance.
The current issue of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses these concerns with two freely accessible protocols: one for inserting 'reporter' proteins into cells to monitor what's going on inside, and another for maintaining the cells under a microscope for long-term observation.
The first protocol, available at
A second freely available protocol
(http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4660) outlines the construction and use of an enclosed microscope chamber. This device is designed to optimize the culture environment for cells--allowing them to grow as they would normally--while optimizing the conditions for viewing them by microscopy. This enables researchers to monitor the cells under a microscope for long time periods (in excess of four days), during which the cells may divide multiple times and cycle through nearly all cellular functions. When coupled with the latest in image-analysis software, this technique allows researchers to gain a long-term perspective on events in the lives of cells.
Maria Smit | EurekAlert!
A novel synthetic antibody enables conditional “protein knockdown” in vertebrates
20.08.2018 | Technische Universität Dresden
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
There are currently great hopes for solid-state batteries. They contain no liquid parts that could leak or catch fire. For this reason, they do not require cooling and are considered to be much safer, more reliable, and longer lasting than traditional lithium-ion batteries. Jülich scientists have now introduced a new concept that allows currents up to ten times greater during charging and discharging than previously described in the literature. The improvement was achieved by a “clever” choice of materials with a focus on consistently good compatibility. All components were made from phosphate compounds, which are well matched both chemically and mechanically.
The low current is considered one of the biggest hurdles in the development of solid-state batteries. It is the reason why the batteries take a relatively long...
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
20.08.2018 | Life Sciences
20.08.2018 | Information Technology
20.08.2018 | Power and Electrical Engineering