Research published in Nature Genetics today describes 381 proteins present in sperm of the fruit fly, Drosophila melanogaster. Whilst more proteins may be identified as research progresses, this study marks the first substantial ’whole-cell’ characterisation of the protein components of a higher eukaryotic cell (a cell in which all the genetic components are contained within a nucleus).
This so-called ‘proteome’ contains everything the sperm needs to survive and function correctly, and scientists can use it to investigate the factors that make some sperm more successful than others.
Around half of the genes of the fruit fly sperm proteome have comparable versions in humans and mice, making it a useful model for studying male infertility in mammals.
By comparing the sperm proteome of the fruit fly with other species, scientists will also be able to rewind evolution and work out the core sperm proteome – the most basic constituents a sperm needs for sexual reproduction. This will shed light on how sex itself evolved.
“This is the first catalogue of sperm proteins for any organism, and it offers a tantalising glimpse into how we might begin to answer some of biology’s most fundamental questions,” said Dr Tim Karr from the University of Bath who led the study.
“Amazingly we know very little about what is in a sperm, which probably explains why we don’t really understand sex, let alone how it evolved.
“Before we catalogued the sperm proteome, we only knew a few specific proteins in the Drosophila sperm.
“Being able to compare the structure and content of the proteomes of sperm from different species should help us understand the evolution and origin of sperm.
“We now know of at least 381, which is a greater than 50-fold increase in our knowledge base. Now that we have identified them, we should be able to study the function of all of these.”
Proteins carry out an immense range of functions, from forming structural materials to catalysing chemical reactions, so knowing exactly what proteins are in sperm is a great step forward in understanding.
The research involved purifying fruit fly sperm and developing methods to study their protein content. Previous estimates for the protein content of sperm were based on counts of proteins separated into ‘spots’ on a special gel matrix. However, these only identify the total number of proteins in sperm – rather than identifying the specific identity of each protein constituent
“The sperm proteome provides a basis for studying the critical functional components of sperm required for motility, fertilisation and possibly early embryo development,” said Dr Steve Dorus, also from the University of Bath, who collaborated with Dr Karr on the project.
“It should be a valuable tool in the study of infertility as more targeted studies can now be established in model organisms.
“Furthermore, having a comprehensive catalogue of proteins to compare between different species will reveal how natural selection has impacted sperm evolution.
“We can start to look for the ‘core’ sperm proteome - that is, the most basic required constituents of sperm. This will not only shed light on the evolutionary origins of sperm, but may advance our understanding of the evolution of sex itself.”
The research will also help further our understanding of sperm competition – the attributes within a sperm that make one sperm more successful at reaching and fertilising the egg than its peers.
“This question of sperm competition has baffled scientists for years,” said Dr Karr.
“If we can work out what makes one sperm more successful than another, we might be able to apply this knowledge to clinical therapies for the treatment of sperm that are not functioning properly.”
The findings are particularly timely as a variety of research is beginning to highlight the increasingly important role of sperm.
Scientists are discovering that as well as carrying the DNA that spells out the male’s contribution to a new life, sperm carries RNA and proteins which have a direct influence on fertilisation and embryo development.
Professor Geoff Parker, Derby Professor of Zoology at the University of Liverpool, said: "This paper provides a remarkable, pioneering analysis of the molecular basis of sperm form and function by identifying 381 proteins of the Drosophila melanogaster sperm proteome, including mitochondrial, metabolic and cytoskeletal proteins.
“Their work has great relevance to current debate on the evolutionary underpinnings of sperm characteristics, and may have application to mammalian sperm function. The Drosophila sperm proteins show substantial homology with the axoneme accessory structure of mouse sperm.”
Professor Manyuan Long, Professor of Genetics & Evolution at the University of Chicago, said: “This is a milestone in the understanding of genomic distribution of male specific proteins. I marvel at their tremendous efforts and great successes.”
The research is funded by the Royal Society and the National Science Foundation with additional support from the Biotechnology & Biological Sciences Research Council and the National Institutes of Health.
Andrew McLaughlin | alfa
Climate Impact Research in Hannover: Small Plants against Large Waves
17.08.2018 | Leibniz Universität Hannover
First transcription atlas of all wheat genes expands prospects for research and cultivation
17.08.2018 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
17.08.2018 | Physics and Astronomy
17.08.2018 | Information Technology
17.08.2018 | Life Sciences