Rapid and accurate diagnosis is thus vital. A team from the Swiss Federal Institute of Technology (ETH) in Zürich, the Swiss Tropical Institute, and the University of Bern has now developed a new immunological approach that can be used to specifically recognize anthrax spores.
A number of tests for the diagnosis of anthrax already exist, including some highly accurate but also extremely complex, time-consuming, and expensive genetic methods. In contrast, immunological tests are very simple; however, it has not yet been possible to develop a truly reliable immunoassay. The similarity of the anthrax spore surface to the spores of other bacteria that commonly occur in humans has been a major problem: previous anthrax antibodies were not sufficiently specific.
Some time ago, a special carbohydrate consisting of four sugar components was discovered on the surface of anthrax spores. This carbohydrate contains a sugar component that occurs nowhere else and has been named anthrose. Peter H. Seeberger and his team targeted this carbohydrate as their point of attack.
In order to produce antibodies against a molecule, one first needs a large enough amount of the molecule in question, or antigen. However, it is exceptionally difficult to isolate a carbohydrate bound to the surface of a cell in its pure form. Seeberger and his team thus chose an alternative route: they synthesized the carbohydrate in the laboratory, attached it to a special “carrier” protein and injected this compound into mice. The carrier protein stimulated an immunological reaction, which is normally rather weak for carbohydrate antigens. The researchers were then able to obtain monoclonal antibodies from these immunized mice.
These antibodies were found to bind very specifically to anthrax spores; in contrast, they do not react to bacteria closely related to Bacillus anthracis. “Our results demonstrate that small differences in the carbohydrates on cell surfaces can be used to obtain specific immune reagents,” says Seeberger. “Our new antibodies will be used as the basis for highly sensitive anthrax diagnosis and will contribute to the development of new therapeutic approaches.”
Peter H. Seeberger | alfa
Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View
22.06.2018 | University of Sussex
New cellular pathway helps explain how inflammation leads to artery disease
22.06.2018 | Cedars-Sinai Medical Center
In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.
Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...
Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...
Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.
Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...
The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.
Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.
An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.
Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...
13.06.2018 | Event News
08.06.2018 | Event News
05.06.2018 | Event News
22.06.2018 | Materials Sciences
22.06.2018 | Earth Sciences
22.06.2018 | Life Sciences