Artificial replication
A collaboration of researchers from Harvard Medical School and the University of Virginia, led by Dr. Anindya Dutta, has created an artificial mammalian origin of replication that will facilitate the future study of mammalian DNA replication.
Dr. Dutta and colleagues recruited known mammalian replication initiation factors (either ORC or CDC6) to a defined GAL4 DNA-binding site on a plasmid, demonstrating that replication initiation factor recruitment is sufficient to specify a DNA replication origin.
The researchers have extended the classic transcription factor reporter assay to work for any eukaryotic replication initiation factor. The artificial mammalian replication origin will enable scientists to explore the mechanism of replication initiation, as well as “provide a new direction for creating vectors for gene therapy that are less mutagenic than current integrating vectors and that do not require viral proteins,” explains Dr. Dutta.
Media Contact
More Information:
http://www.cshl.eduAll latest news from the category: Life Sciences and Chemistry
Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.
Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.
Newest articles
High-energy-density aqueous battery based on halogen multi-electron transfer
Traditional non-aqueous lithium-ion batteries have a high energy density, but their safety is compromised due to the flammable organic electrolytes they utilize. Aqueous batteries use water as the solvent for…
First-ever combined heart pump and pig kidney transplant
…gives new hope to patient with terminal illness. Surgeons at NYU Langone Health performed the first-ever combined mechanical heart pump and gene-edited pig kidney transplant surgery in a 54-year-old woman…
Biophysics: Testing how well biomarkers work
LMU researchers have developed a method to determine how reliably target proteins can be labeled using super-resolution fluorescence microscopy. Modern microscopy techniques make it possible to examine the inner workings…
