Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Quick and easy: new ELISA for plant virus detection yields results after two hours

23.06.2016

Scientists at the Leibniz Institute DSMZ have developed an extremely timesaving immunoassay variant. Using the classic ELISA, results are obtained only after up to two days. However, the new ‘B-Fast ELISA’ yields reliable results already after about two hours. Nevertheless, the B-Fast ELISA equals the classic methods with regard to sensitivity, specificity and the possibility of semi-quantitative analysis. At the moment, the DSMZ offers the B-Fast ELISA for the detection of ten different plant viruses, for example the Tomato spotted wilt virus or the Maize chlorotic mottle virus, which belong to the economically most important viruses worldwide.

The plant virologists Dr. Wulf Menzel and Dr. Stephan Winter from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures have developed a simplified, much faster variant of the enzyme-linked immunosorbent assay (ELISA) for the detection of plant viruses, thereby cooperating with the Australian biotechnology company TGR BioSciences.

Using the classic ELISA, results are obtained only after up to two days. However, the new ‘B-Fast ELISA’ yields reliable results already after about two hours.

“Our rapid test comprises much fewer steps and much shorter incubation times than the classic ELISA variants used up to now, so that the amount of work is considerably reduced,” Wulf Menzel explains. “Nevertheless, the B-Fast ELISA equals the classic methods with regard to sensitivity, specificity and the possibility of semi-quantitative analysis”.

The available kit contains 12 separate 8-well strips, so that low sample numbers can be tested without having to use an entire plate. “Thus, this ‘Friday afternoon ELISA’ is very convenient if the results are needed within a short time or if low sample numbers have to be tested frequently,” Wulf Menzel says.

The ELISA is the commonly used method for the routine detection of plant viruses. The new B-Fast ELISA variant, which is now available in plant pathogen diagnostics for the first time, is based on a technology developed by TGR BioSciences (CaptSure™ technology).

Its special feature is the coupling of the primary antibody with a peptide which can bind to another antibody immobilized on the ELISA plate. Thus, the incubation of primary and secondary antibody together with the sample can occur at the same time on the plate, and the entire complex is immobilized. After a washing step, detection by an enzymatic color reaction takes place.

At the moment, the DSMZ offers the B-Fast ELISA for the detection of ten different plant viruses, for example the Tomato spotted wilt virus or the Maize chlorotic mottle virus, which belong to the economically most important viruses worldwide. Moreover, the scientists are working on the expansion of the method in cooperation with TGR BioSciences, so that more viruses can be detected in the future.

Scientific contact
Dr. Wulf Menzel
Department plant viruses
Phone: 0531 2616-402
E-Mail: plantvirus@dsmz.de

About Leibniz Institute DSMZ
The Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures GmbH is a Leibniz Association institution. Offering comprehensive scientific services and a wide range of biological materials it has been a partner for research and industry organizations worldwide for decades. DSMZ is one of the largest biological resource centers of its kind to be compliant with the internationally recognized quality norm ISO 9001:2008. As a patent depository, DSMZ currently offers the only option in Germany of accepting biological materials according to the requirements of the Budapest Treaty. The second major function of DSMZ, in addition to its scientific services, is its collection-related research. The Brunswick (Braunschweig), Germany, based collection has existed for 42 years and holds more than 52,000 cultures and biomaterials. DSMZ is the most diverse collection worldwide: In addition to fungi, yeasts, bacteria, and archea, it is home to human and animal cell cultures, plant viruses, and plan cell cultures that are archived and studied there. http://www.dsmz.de

Christian Engel | idw - Informationsdienst Wissenschaft

Further reports about: BioSciences Cell DSMZ biological materials cell cultures plant virus viruses

More articles from Life Sciences:

nachricht Zebrafish's near 360 degree UV-vision knocks stripes off Google Street View
22.06.2018 | University of Sussex

nachricht New cellular pathway helps explain how inflammation leads to artery disease
22.06.2018 | Cedars-Sinai Medical Center

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Superconducting vortices quantize ordinary metal

Russian researchers together with their French colleagues discovered that a genuine feature of superconductors -- quantum Abrikosov vortices of supercurrent -- can also exist in an ordinary nonsuperconducting metal put into contact with a superconductor. The observation of these vortices provides direct evidence of induced quantum coherence. The pioneering experimental observation was supported by a first-ever numerical model that describes the induced vortices in finer detail.

These fundamental results, published in the journal Nature Communications, enable a better understanding and description of the processes occurring at the...

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Rapid water formation in diffuse interstellar clouds

25.06.2018 | Physics and Astronomy

Using tree-fall patterns to calculate tornado wind speed

25.06.2018 | Earth Sciences

'Stealth' material hides hot objects from infrared eyes

25.06.2018 | Power and Electrical Engineering

VideoLinks
Science & Research
Overview of more VideoLinks >>>