Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Now you can 'build your own' bio-bot

14.02.2017

I'll bet you don't have one of these at home.

For the past several years, researchers at the University of Illinois at Urbana-Champaign have been developing a class of walking "bio-bots" powered by muscle cells and controlled with electrical and optical pulses. Now, Rashid Bashir's research group is sharing the recipe for the current generation of bio-bots. Their how-to paper is the cover article in Nature Protocols.


This is a schematic of a bio-bot: Engineered skeletal muscle tissue is coupled to a 3-D printed flexible skeleton. Optical stimulation of the muscle tissue, which is genetically engineered to contract in response to blue light, makes the bio-bot walk across a surface in the direction of the light.

Credit: Janet Sinn-Hanlon, University of Illinois

"The protocol teaches every step of building a bio-bot, from 3D printing the skeleton to tissue engineering the skeletal muscle actuator, including manufacturers and part numbers for every single thing we use in the lab," explained Ritu Raman, now a postdoctoral fellow in the Department of Bioengineering and first author of the paper, "A modular approach to the design, fabrication, and characterization of muscle-powered biological machines."

"This protocol is essentially intended to be a one-stop reference for any scientist around the world who wants to replicate the results we showed in our PNAS 2016 and PNAS 2014 papers, and give them a framework for building their own bio-bots for a variety of applications," Raman said.

As stated in the paper, "Biological machines consisting of cells and biomaterials have the potential to dynamically sense, process, respond, and adapt to environmental signals in real time." This can result in exciting possibilities where these "systems could one day demonstrate complex behaviors including self-assembly, self-organization, self-healing, and adaptation of composition and functionality to best suit their environment."

Bashir's group has been a pioneer in designing and building bio-bots, less than a centimeter in size, made of flexible 3D printed hydrogels and living cells. In 2012, the group demonstrated bio-bots that could "walk" on their own, powered by beating heart cells from rats. However, heart cells constantly contract, denying researchers control over the bot's motion.

"The purpose of the paper was to provide the detailed recipes and protocols so that others can easily duplicate the work and help to further permeate the idea of 'building with biology'--so that other researchers and educators can have the tools and the knowledge to build these bio-hybrid systems and attempt to address challenges in health, medicine, and environment that we face as a society," stated Rashid Bashir, a Grainger Distinguished Chair in Engineering and head of the Department of Bioengineering at Illinois.

"The 3D printing revolution has given us the tools required to 'build with biology' in this way." Raman said. "We re-designed the 3D-printed injection mold to produce skeletal muscle 'rings' that could be manually transferred to any of a wide variety of bio-bot skeletons. These rings were shown to produce passive and active tension forces similar to those generated by muscle strips.

"Using optogenetics techniques, we worked with collaborators at MIT to genetically engineer a light-responsive skeletal muscle cell line that could be stimulated to contract by pulses of 470-nm blue light," Raman added. "The resultant optogenetic muscle rings were coupled to multi-legged bio-bot skeletons with symmetric geometric designs. Localized stimulation of contraction, rendered possible by the greater spatiotemporal control of light stimuli over electrical stimuli, was used to drive directional locomotion and 2D rotational steering."

###

In addition to Bashir and Raman, Caroline Cvetkovic, a recent graduate student in bioengineering and now a post-doctoral fellow was a co-author of the paper. Work on the bio-bots was conducted at the Micro + Nanotechnology Lab at Illinois.

Rashid Bashir | EurekAlert!

Further reports about: 3D beating heart blue light heart cells living cells muscle cells skeletal muscle

More articles from Life Sciences:

nachricht Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY

nachricht NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Future electronic components to be printed like newspapers

A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.

The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...

Im Focus: First evidence on the source of extragalactic particles

For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.

To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...

Im Focus: Magnetic vortices: Two independent magnetic skyrmion phases discovered in a single material

For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.

Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...

Im Focus: Breaking the bond: To take part or not?

Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.

A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...

Im Focus: New 2D Spectroscopy Methods

Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.

"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Leading experts in Diabetes, Metabolism and Biomedical Engineering discuss Precision Medicine

13.07.2018 | Event News

Conference on Laser Polishing – LaP: Fine Tuning for Surfaces

12.07.2018 | Event News

11th European Wood-based Panel Symposium 2018: Meeting point for the wood-based materials industry

03.07.2018 | Event News

 
Latest News

A smart safe rechargeable zinc ion battery based on sol-gel transition electrolytes

20.07.2018 | Power and Electrical Engineering

Reversing cause and effect is no trouble for quantum computers

20.07.2018 | Information Technology

Princeton-UPenn research team finds physics treasure hidden in a wallpaper pattern

20.07.2018 | Materials Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>