Scientists at the Leibniz-Institute of Plant Biochemistry (IPB) in Halle/Saale (Germany) have fully elucidated the biosynthesis of carnosic acid. This discovery allowed the plant researchers around Prof. Alain Tissier to produce the economically valuable plant material by biotechnological means in yeast cells. The project was published in the renowned journal Nature Communications.
Carnosic acid is a natural antioxidant that is found in the leaves of rosemary and sage. It is used worldwide as a preservative and flavor in meat products, oils, fats, sauces and animal feed. Carnosic acid, for which the demand is steadily increasing, is still extracted from rosemary plants, which grow slowly.
Carnosic acid is still obtained from rosemary. However, biotechnological production processes could be developed soon.
Dried leaves of sage or rosemary contain at most 2.5 percent of carnosic acid, necessitating a large amount of plant material to ensure the production of the antioxidant on industrial scale. Furthermore, the complex structure of carnosic acid makes an industrial synthetic process unrealistic.
The biosynthesis of carnosic acid within the plant takes place in several reaction steps, which are catalyzed by different enzymes. The enzyme that catalyzes the last step of the reaction chain had not yet been discovered. This knowledge-gap has now been closed by the IPB researchers.
They discovered an additional, previously unknown intermediate and also new enzymes, which were described and characterized by them. With the knowledge of all involved reaction partners, the scientists were able to introduce the genes coding for the corresponding enzymes into yeast cells and make them produce carnosic acid. This is the first step in the development of a biotechnological production process for the antioxidant.
Carnosic acid is also the starting material for the biosynthesis of many other phenolic diterpenes, which act as bioactive substances against inflammation, cancer and various neurodegenerative diseases.
Also for this reason, it will be interesting to produce carnosic acid in the future with biotechnology-based processes and thus independently of climate fluctuations, soil quality and harvest yields.
Ulschan Scheler, Wolfgang Brandt, Andrea Porzel, Kathleen Rothe, David Manzano, Dragana Bozic, Dimitra Papaefthimiou, Gerd Ulrich Balcke, Anja Henning, Swanhild Lohse, Sylvestre Marillonnet, Angelos K. Kanellis, Albert Ferrer & Alain Tissier. Elucidationof the bioynthesis of carnosic acid and its reconstitution in yeast. Nature Communications 7: 12942, doi:10.1038/ncomms12
Prof. Alain Tissier
Leibniz Institute of Plant Biochemistry
Tel.: +49 345 5582 1500
Dipl.Biol. Sylvia Pieplow | idw - Informationsdienst Wissenschaft
Colorectal cancer risk factors decrypted
13.07.2018 | Max-Planck-Institut für Stoffwechselforschung
Algae Have Land Genes
13.07.2018 | Julius-Maximilians-Universität Würzburg
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
Ultra-short, high-intensity X-ray flashes open the door to the foundations of chemical reactions. Free-electron lasers generate these kinds of pulses, but there is a catch: the pulses vary in duration and energy. An international research team has now presented a solution: Using a ring of 16 detectors and a circularly polarized laser beam, they can determine both factors with attosecond accuracy.
Free-electron lasers (FELs) generate extremely short and intense X-ray flashes. Researchers can use these flashes to resolve structures with diameters on the...
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
13.07.2018 | Event News
13.07.2018 | Materials Sciences
13.07.2018 | Life Sciences