Researchers at the Stowers Institute for Medical Research have solved the three-dimensional structure of a complex that is essential for the correct sorting of chromosomes into eggs and sperm during reproductive cell division or meiosis.
When this structure, called the synaptonemal complex, doesn't assemble properly in the cell, it can lead to chromosomal abnormalities, miscarriages, and birth defects.
Since the synaptonemal complex was first discovered in 1956, researchers have been trying to identify its many moving parts and how they fit together. Their efforts have been limited by the laws of physics: the structure is too small to be visualized by even the most high-power microscopes.
Now, Stowers researchers, including Cori Cahoon, Zulin Yu, Ph.D., and Yongfu Wang, Ph.D., have married two advanced microscopy techniques - one that enlarges samples to several times their original size and another that uses computers to capture what the human eye cannot see - to render the likeness of this enigmatic structure. Their findings, reported in the July 31, 2017 online early edition of the Proceedings of the National Academy of Sciences, were not at all what they expected.
"The structure was so much more complicated and beautiful than we ever imagined," says R. Scott Hawley, Ph.D., an investigator at the Stowers Institute and senior author of the study. "We thought it just looked like a railroad track, but we discovered that it is actually more like two railroad tracks, one stacked on top of the other. That changes the way we think about this structure and what it does."
In meiosis, the cell copies all its chromosomes, pairs them up, and then divides them into eggs or sperm. This carefully choreographed process is helped along by the synaptonemal complex, an assembly of proteins that forms between the paired homologous chromosomes and keeps them properly aligned and in position. Errors during meiosis are a leading cause of miscarriages in humans.
For decades, researchers working in yeast, flies, worms, and even some mammals have tried to get a close look at the evolutionarily conserved synaptonemal complex structure. Then last year, Yu and Wang, researchers in the Stowers Microscopy and Histology facilities, respectively, learned of a new technique called expansion microscopy that allows structures to be visualized with nanoscale precision. They contacted Cahoon, a predoctoral researcher in Hawley's lab who was studying the synaptonemal complex, and suggested they give it a try.
"She was willing to take a chance and do the experiments, even though they were risky. It paid off big time," says Hawley. "What they accomplished was a technical tour de force."
First, the team gathered samples of the synaptonemal complex from dissected fruit fly ovaries. They embedded these samples in a special type of gel, added liquid, and watched the samples expand fourfold. Then, the researchers studied the super-sized samples using the super-resolution technique called structured illumination microscopy.
What they found was startling: the structure was divided into two identical layers, which had been indistinguishable at lower resolution. Basically, the synaptonemal complex connects two homologous chromosomes, each with two sister chromatids stacked on one another. The researchers showed that this complex uses a two-tiered approach to connect the two upper chromatids and the two lower chromatids separately. They created a computer model of the structure showing coils of DNA connected by two sets of railroad tracks, which are made up of the four known proteins that are part of the synaptonemal complex. Although in this study the researchers looked at only four proteins, many more unknown proteins likely reside in the structure as well, so there are still more details to fill in.
"This was a really incredible insight, a technical leap using this new methodology of expansion microscopy and merging it with structured illumination to create a way to look at a structure that hasn't been resolved before," says Hawley. "There is a lot more we can learn. The deeper we dive into structure, the more complexity we see, the more amazing the structure becomes. Structure provides so much insight into function."
Other contributors from the Stowers Institute include Fengli Guo, Ph.D., Jay R. Unruh, Ph.D., and Brian D. Slaughter, Ph.D.
The work was funded by the Stowers Institute for Medical Research and R. Scott Hawley is an American Cancer Society Professor.
Lay Summary of Findings
Errors in meiosis, the process of cell division that gives rise to eggs and sperm, are the leading cause of miscarriage in humans. In a study published in the July 31, 2017 online early edition of the Proceedings of the National Academy of Sciences, Stowers Institute scientists solve the three-dimensional structure of a protein complex that is essential to meiosis. By merging two advanced microscopy techniques, the researchers discovered that this structure, known as the synaptonemal complex, looks like two sets of railroad tracks stacked on top of each other. According to Investigator R. Scott Hawley, Ph.D., the study represents a technical leap and provides a new window on the way this critical complex works to keep meiosis moving smoothly.
About the Stowers Institute for Medical Research
The Stowers Institute for Medical Research is a non-profit, basic biomedical research organization dedicated to improving human health by studying the fundamental processes of life. Jim Stowers, founder of American Century Investments, and his wife, Virginia, opened the Institute in 2000. Currently, the Institute is home to about 500 researchers and support personnel, over 20 independent research programs, and more than a dozen technology development and core facilities. Learn more about the Institute at http://www.
Kim Bland | EurekAlert!
Nanotubes built from protein crystals: Breakthrough in biomolecular engineering
15.11.2018 | Tokyo Institute of Technology
Insect Antibiotic Provides New Way to Eliminate Bacteria
15.11.2018 | Universität Zürich
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure
Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...
Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly
The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...
09.11.2018 | Event News
06.11.2018 | Event News
23.10.2018 | Event News
15.11.2018 | Earth Sciences
15.11.2018 | Physics and Astronomy
15.11.2018 | Physics and Astronomy