Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Continuous Manufacturing Platform for Commercially Relevant Viral Gene Therapy Vectors

17.04.2020

On October 1st 2019, the spin-off project “ContiVir” officially started research and development activities at the Max Planck Institute (MPI) in Magdeburg. The two-year EXIST-Transfer of Research project is jointly funded by the European Union, the German Ministry for Economic Affairs and Energy, and the Max Planck Society. The project will focus on the development of a continuous manufacturing platform for commercially relevant viral gene therapy vectors. The grant is the result of the combined doctoral research of Dr.-Ing. Felipe Tapia and Dr.-Ing. Pavel Marichal, scientists of the Bioprocessing Engineering group headed by Prof. Dr.-Ing. Udo Reichl.

Gene therapies have shown the promise to become the next medical revolution for combating a wide variety of currently untreatable diseases. The most common delivery method for gene therapy are with viral vectors: genetically engineered viruses used as vehicles to introduce genetic material into the patient’s cells to replace defective genes by healthy ones. Currently, gene therapies cost from hundreds of thousands to millions of euros per patient.


ContiVir project will focus on the development of a continuous manufacturing platform for commercially relevant viral gene therapy vectors

ContiVir / Felipe Tapia und Pavel Marichal

A significant increase in the demand for viral gene therapy vectors is expected in the future, evidenced by both the market authorization of the first gene therapies in Europe and the US in recent years, and more than a thousand treatments in clinical phase. It is estimated that nowadays the demand for viral gene therapy vectors is five times higher than the current supply.

A major bottleneck in the supply of viral vectors is requirement for high dose titers and inefficient production techniques based on batch technologies meant for laboratory use. The latter are incredibly difficult to scale-up for commercial production and require large amounts of time and labor.

The EXIST-Transfer of Research project started aims to tackle some of the manufacturing issues described above by combining two disruptive new technologies established by the Bioprocess Engineering group of the MPI Magdeburg.

Tubular Bioreactor combined with a Membrane-Based Chromatography Technique

The first technology is a continuous tubular bioreactor developed by Felipe Tapia in the Upstream Processing team of PD. Dr. Yvonne Genzel. This bioreactor is the first fully continuous system for the production of virus particles and has a footprint twenty times smaller than current batch systems. Additionally, continuous processes have higher productivity than batch cultures for large volumes, consume less energy, and require less labor.
original

The viral vectors produced in the tubular bioreactor will be purified with a membrane-based chromatography technique developed by Pavel Marichal in the Downstream Processing team of Prof. Dr. Michael Wolff (now at the Technische Hochschule Mittelhessen, University of Applied Sciences in Giessen, Germany).

The purification method, named steric exclusion chromatography (SXC), can be used to concentrate and purify a wide variety of viruses with high product yields using very similar process conditions supporting short process development. SXC uses low-cost materials and is easily scalable.

Both technologies will be integrated in a fully continuous manufacturing process that has the potential to meet the future demand of viral vectors to make gene therapies widely available to the general population.

This manufacturing platform can also be used to support the production of viral vaccines in large quantities during pandemics, such as the current coronavirus outbreak that is considered by many countries as the worst crisis since World War 2.

The project will be carried out in the laboratories of the MPI Magdeburg and the Otto von Guericke University Magdeburg.

Weitere Informationen:

https://www.mpi-magdeburg.mpg.de/3925352/2020-04-15-contivir-exist-projekt?c=301...
https://www.contivir.com/

Gabriele Ebel M.A. | Max-Planck-Institut für Dynamik komplexer technischer Systeme Magdeburg

More articles from Life Sciences:

nachricht Rising water temperatures could endanger the mating of many fish species
03.07.2020 | Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung

nachricht Moss protein corrects genetic defects of other plants
03.07.2020 | Rheinische Friedrich-Wilhelms-Universität Bonn

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Electrons in the fast lane

Solar cells based on perovskite compounds could soon make electricity generation from sunlight even more efficient and cheaper. The laboratory efficiency of these perovskite solar cells already exceeds that of the well-known silicon solar cells. An international team led by Stefan Weber from the Max Planck Institute for Polymer Research (MPI-P) in Mainz has found microscopic structures in perovskite crystals that can guide the charge transport in the solar cell. Clever alignment of these "electron highways" could make perovskite solar cells even more powerful.

Solar cells convert sunlight into electricity. During this process, the electrons of the material inside the cell absorb the energy of the light....

Im Focus: The lightest electromagnetic shielding material in the world

Empa researchers have succeeded in applying aerogels to microelectronics: Aerogels based on cellulose nanofibers can effectively shield electromagnetic radiation over a wide frequency range – and they are unrivalled in terms of weight.

Electric motors and electronic devices generate electromagnetic fields that sometimes have to be shielded in order not to affect neighboring electronic...

Im Focus: Gentle wall contact – the right scenario for a fusion power plant

Quasi-continuous power exhaust developed as a wall-friendly method on ASDEX Upgrade

A promising operating mode for the plasma of a future power plant has been developed at the ASDEX Upgrade fusion device at Max Planck Institute for Plasma...

Im Focus: ILA Goes Digital – Automation & Production Technology for Adaptable Aircraft Production

Live event – July 1, 2020 - 11:00 to 11:45 (CET)
"Automation in Aerospace Industry @ Fraunhofer IFAM"

The Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM l Stade is presenting its forward-looking R&D portfolio for the first time at...

Im Focus: AI monitoring of laser welding processes - X-ray vision and eavesdropping ensure quality

With an X-ray experiment at the European Synchrotron ESRF in Grenoble (France), Empa researchers were able to demonstrate how well their real-time acoustic monitoring of laser weld seams works. With almost 90 percent reliability, they detected the formation of unwanted pores that impair the quality of weld seams. Thanks to a special evaluation method based on artificial intelligence (AI), the detection process is completed in just 70 milliseconds.

Laser welding is a process suitable for joining metals and thermoplastics. It has become particularly well established in highly automated production, for...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

International conference QuApps shows status quo of quantum technology

02.07.2020 | Event News

Dresden Nexus Conference 2020: Same Time, Virtual Format, Registration Opened

19.05.2020 | Event News

Aachen Machine Tool Colloquium AWK'21 will take place on June 10 and 11, 2021

07.04.2020 | Event News

 
Latest News

Rising water temperatures could endanger the mating of many fish species

03.07.2020 | Life Sciences

Risk of infection with COVID-19 from singing: First results of aerosol study with the Bavarian Radio Chorus

03.07.2020 | Studies and Analyses

Efficient, Economical and Aesthetic: Researchers Build Electrodes from Leaves

03.07.2020 | Power and Electrical Engineering

VideoLinks
Science & Research
Overview of more VideoLinks >>>