“It’s a basic discovery, but with implications for understanding disease,” said Jonathan Scholey, professor of molecular and cellular biology at UC Davis and senior author of the study. Understanding how cilia are assembled and function can help scientists understand how conditions such as polycystic kidney disease and some growth and development disorders arise.
Cilia are built from bundles of microtubules made of a protein called tubulin. Scholey’s team discovered how two subunits of tubulin are winched into place by a type of protein motor belonging to a family of proteins called kinesins.
Scholey’s laboratory works with the soil roundworm Caenorhabditis elegans, whose cilia are essentially the same as those of humans and other mammals. Postdoctoral scholar Limin Hao, Scholey and their colleagues screened a collection of worms for those with mutations that affected the cilia.
They found two genes which, when mutated, caused worms to lose the tips of their cilia. Both genes turned out to be subunits of tubulin that are assembled into different parts of the microtubule: one is found all along the microtubule, and the other is concentrated at the tip.
The UC Davis team used a combination of microscopy, molecular biology and computer modeling to study these two proteins. They found that both are moved into position by so-called kinesin-2 motors.
At one time, researchers had seen cilia as purely for movement, either moving a swimming cell through a fluid or moving fluid and suspended particles over the cell’s surface, Scholey said.
But in the late 1990s, researchers discovered that cilia were also involved in detecting signaling molecules that control gene expression and cell behavior. This signaling is vital for coordinating cell growth and the orderly development of tissues, for example in establishing left/right asymmetry in developing embryos.
“Recent work shows that cilia are ubiquitous in signaling,” Scholey said. In earlier work, Scholey’s lab linked a defect in the kinesins that assemble cilia to Bardet-Biedl disease, which causes blindness, kidney disease and learning difficulties.
Coauthors of the paper are: Melanie Thein, Ingrid Brust-Mascher, Gul Civelekoglu-Scholey and Seyda Acar, all at UC Davis; Yun Lu and Shai Shaham, Rockefeller University, New York; and Bram Prevo, Vrije Universiteit Amsterdam, The Netherlands, who was a visiting scholar in Scholey’s laboratory. Hao is now a researcher at Harvard Medical School and Thein is now a writer/editor at the UC Davis Cancer Center. The work was funded by grants from the National Institutes of Health.Media contact(s):
Andy Fell, UC Davis News Service, (530) 752-4533, email@example.com
Andy Fell | EurekAlert!
Nonstop Tranport of Cargo in Nanomachines
20.11.2018 | Max-Planck-Institut für molekulare Zellbiologie und Genetik
Researchers find social cultures in chimpanzees
20.11.2018 | Universität Leipzig
Max Planck researchers revel the nano-structure of molecular trains and the reason for smooth transport in cellular antennas.
Moving around, sensing the extracellular environment, and signaling to other cells are important for a cell to function properly. Responsible for those tasks...
Researchers at the University of New Hampshire have captured a difficult-to-view singular event involving "magnetic reconnection"--the process by which sparse particles and energy around Earth collide producing a quick but mighty explosion--in the Earth's magnetotail, the magnetic environment that trails behind the planet.
Magnetic reconnection has remained a bit of a mystery to scientists. They know it exists and have documented the effects that the energy explosions can...
Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.
Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...
Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.
In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...
On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.
19.11.2018 | Event News
09.11.2018 | Event News
06.11.2018 | Event News
20.11.2018 | Life Sciences
20.11.2018 | Life Sciences
20.11.2018 | Physics and Astronomy