In a pioneering study, scientists in Jena, Borstel, Aberdeen and London have discovered a toxin in the fungus Candida albicans, which plays a crucial role during human mucosal infection. Their discovery is has now been published in the journal Nature.
Throughout evolution, pathogens have come up with many tricks to infect and damage their hosts: viruses capture whole cells and turn them into factories for their own replication until the cells are exhausted and die. Infectious bacteria produce multiple molecules that can manipulate the host cell’s metabolism or simply destroy it. But what about human pathogenic fungi?
Candida albicans attached to the oral mucosa as a yeast cell and forms a filamentous hypha. The hypha produces Candidalysin, which has now been discovered by a team of German and British researchers
Holland, Özel, Zakikhany, Hube
It is well known that certain fungi produce poisons which, if accidentally consumed, can result in sickness or even death. However, up until now, production of host cell-destroying toxins by the microscopic fungi that can infect us has never been shown. For decades, scientists have been looking for the molecules that are directly responsible for tissue damage during the course of fungal diseases.
However, they did not find toxins in pathogenic fungi, which directly damages their infected hosts, and distinguishes these dangerous species from their harmless relatives. This is regrettable, since such knowledge would have been of great value for understanding human fungal infections and for taking therapeutic countermeasures.
Hence, the discovery by a German-British team of researchers came as quite a surprise. Microbiologists from Jena, Borstel, Aberdeen and London succeeded for the first time in identifying a real toxin in Candida albicans – a usually harmless gut-dwelling yeast which frequently causes diseases such as thrush.
Candidalysin, as the new toxin was called, destroys human cells by forming holes in their membranes. The team of scientists elucidated this mechanism using cells of the oral mucosa as a model. Oral infections with Candida albicans commonly occur in HIV patients, but also in very young and elderly people with weakened immune systems.
The initial impulse for the discovery came from Julian Naglik’s research group at King’s CollegeLondon, who has been studying how human oral cells respond to fungal infections. The team of Bernhard Hube at the Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute – (HKI) in Jena, Germany, investigated the interactions between fungus and host on a molecular level and demonstrated that Candidalysin actually damages the host cell.
In addition, the biophysicist Thomas Gutsmann at the Leibniz-Center for Medicine and Biosciences in Borstel and his group studied the precise interaction between the fungal toxin and the cell membrane. Additional researchers in the UK and the USA also contributed data. The discovery of Candidalysin was made possible by a successful combination of individual expertise.
But why did it take researchers several decades of intensive investigations to find the crucial molecule? Similar toxic compounds – peptides – have long been known from other microbial pathogens. Here, the trick is that Candida albicans initially produces a much larger molecule – a polypeptide. The gene encoding for this polypeptide has long been known; however, its function remained elusive. Only now, using state-of-the-art analytics and building on recent insights, Naglik, Hube and their colleagues could successfully detect and characterize the small molecule.
They realized that the polypeptide is cut into small pieces inside the fungus by a certain enzyme, but only one of these peptide fragments is the newly discovered toxin Candidalysin. The scientists chose this particular name because the peptide contributes to the destruction – the “lysis” – of the cell. From a harmless precursor, the dangerous toxin is only produced when it is actually required by the pathogen.
The production of this toxin is tightly linked to a morphological switch, which is crucial for disease. Candida albicans grows in two different forms: either as an egg-shaped yeast cell or as a filamentous hyphal form. In the human body, small numbers of yeast cells are harmless and our immune systems realizes that it does not face any (immediate) danger. However, when invasive filaments are formed, they release Candidalysin, and it is the activity of the toxin itself that is recognized, acting as a “red alert” signal. Therefore, the immune system has learned to recognize Candidalysin in order to discriminate between harmless yeasts and invasive, toxin-producing “dangerous” filaments. “This is an excellent example of co-evolution”, says Bernhard Hube, Professor at Friedrich Schiller University Jena and department head at HKI.
“The pathogen generates a toxin to harm its host and the host quickly senses the toxin and initiates countermeasures”. The biological function of the toxin during normal growth, when the fungus lives harmlessly on the mucosa currently remains unknown.
“These ground-breaking results in fungal infection biology clearly show that in addition to copious amounts of tenacity, the most important factor for scientific success is an excellent power of observation”, states Hube. “Every small result and every observation constantly has to be re-evaluated and re-interpreted until the molecular reality becomes visible. The outstanding international collaboration within our team of researchers has been crucial for our success. For the scientists from Leibniz Institutes this is their own special contribution to this year’s Leibniz Anniversary.”
The fungus Candida albicans and its toxin Candidalysin will continue to keep scientists from Jena, Borstel and London asking questions for many years to come. How does the toxin interact with the immune system? Does the toxin also have activity against bacteria and does this have any effects on common habitats such as in the human gut? What role do other genetic components of the fungus play during infection?
Moyes DL, Wilson D, Richardson JP, Mogavero S, Tang SX, Wernecke J, Höfs S, Gratacap RL, Robbins J, Runglall M, Murciano C, Blagojevic M, Thavaraj S, Förster TM, Hebecker B, Kasper L, Vizcay G, Iancu SI, Kichik N, Häder A, Kurzai O, Luo T, Krüger T, Kniemeyer O, Cota E, Bader O, Wheeler RT, Gutsmann T, Hube B, Naglik JR (2016) Candidalysin is a fungal peptide toxin critical for mucosal infection. Nature 532, 64–68, doi:10.1038/nature17625. www.nature.com/nature/journal/v532/n7597/full/nature17625.html
Dr. Michael Ramm | idw - Informationsdienst Wissenschaft
Scientists uncover the role of a protein in production & survival of myelin-forming cells
19.07.2018 | Advanced Science Research Center, GC/CUNY
NYSCF researchers develop novel bioengineering technique for personalized bone grafts
18.07.2018 | New York Stem Cell Foundation
A new manufacturing technique uses a process similar to newspaper printing to form smoother and more flexible metals for making ultrafast electronic devices.
The low-cost process, developed by Purdue University researchers, combines tools already used in industry for manufacturing metals on a large scale, but uses...
For the first time ever, scientists have determined the cosmic origin of highest-energy neutrinos. A research group led by IceCube scientist Elisa Resconi, spokesperson of the Collaborative Research Center SFB1258 at the Technical University of Munich (TUM), provides an important piece of evidence that the particles detected by the IceCube neutrino telescope at the South Pole originate from a galaxy four billion light-years away from Earth.
To rule out other origins with certainty, the team led by neutrino physicist Elisa Resconi from the Technical University of Munich and multi-wavelength...
For the first time a team of researchers have discovered two different phases of magnetic skyrmions in a single material. Physicists of the Technical Universities of Munich and Dresden and the University of Cologne can now better study and understand the properties of these magnetic structures, which are important for both basic research and applications.
Whirlpools are an everyday experience in a bath tub: When the water is drained a circular vortex is formed. Typically, such whirls are rather stable. Similar...
Physicists working with Roland Wester at the University of Innsbruck have investigated if and how chemical reactions can be influenced by targeted vibrational excitation of the reactants. They were able to demonstrate that excitation with a laser beam does not affect the efficiency of a chemical exchange reaction and that the excited molecular group acts only as a spectator in the reaction.
A frequently used reaction in organic chemistry is nucleophilic substitution. It plays, for example, an important role in in the synthesis of new chemical...
Optical spectroscopy allows investigating the energy structure and dynamic properties of complex quantum systems. Researchers from the University of Würzburg present two new approaches of coherent two-dimensional spectroscopy.
"Put an excitation into the system and observe how it evolves." According to physicist Professor Tobias Brixner, this is the credo of optical spectroscopy....
13.07.2018 | Event News
12.07.2018 | Event News
03.07.2018 | Event News
20.07.2018 | Power and Electrical Engineering
20.07.2018 | Information Technology
20.07.2018 | Materials Sciences