A UCSB biophysicist and deputy director of the Kavli Institute for Theoretical Physics collaborates with colleagues to describe the geometry of a common cellular structure
Architecture imitates life, at least when it comes to those spiral ramps in multistory parking garages. Stacked and connecting parallel levels, the ramps are replications of helical structures found in a ubiquitous membrane structure in the cells of the body.
Dubbed Terasaki ramps after their discoverer, they reside in an organelle called the endoplasmic reticulum (ER), a network of membranes found throughout the cell and connected to and surrounding the cell nucleus. Now, a trio of scientists, including UC Santa Barbara biological physicist Greg Huber, describe ER geometry using the language of theoretical physics. Their findings appear in print and online in the Oct. 31 issue of Physical Review Letters.
"Our work hypothesizes how the particular shape of this organelle forms, based on the interactions between Terasaki ramps," said Huber, who is deputy director of UCSB's Kavli Institute for Theoretical Physics. "A physicist would like to say there's a reason for the membrane's shape, that it's not just an accident. So by understanding better the physics responsible for the shape, one can start to think about other unsolved questions, including how its form relates to its function and, in the case of disease, to its dysfunction."
The rough ER consists of a number of more or less regular stacks of evenly spaced connected sheets, a structure that reflects its function as the shop floor of protein synthesis within a cell. Until recently, scientists assumed that the connections between adjacent sheets were like wormholes — that is, simple tubes.
Last year, however, it was discovered that these connections are formed by spiral ramps running up through the stack of sheets. According to lead author Jemal Guven of the Universidad Nacional Autónoma de México, this came as a surprise because spiral geometries had never previously been observed in biological membranes.
Attached to the membrane, ribosomes, which serve as the primary site for protein synthesis, dot the ER like cars populating a densely packed parking structure. "The ribosomes have to be a certain distance apart because otherwise they can't synthesize proteins," Huber explained.
"So how do you get as many ribosomes per unit volume as possible but not have them bump up against each other?" Huber asked. "The cell seems to have solved that problem by folding surfaces into layers that are nearly parallel to each other and allow a high density of ribosomes."
Different parts of the ER have different shapes: a network of tubes, a sphere that bounds the nucleus or a set of parallel sheets like the levels of a parking garage. The smooth ER consists of a tubular network of membranes meeting at three-way junctions. These junctions are also the location of lipid (or membrane) synthesis. As new lipids are produced within the smooth ER, they accumulate in these junctions, eventually cleaving apart the tubes meeting there.
In the rough ER, the parallel surfaces or stacks are connected by Terasaki spiral ramps. In some cases, one ramp is left-handed and the other right-handed — the parking-garage geometry — which is what Terasaki and colleagues (including Huber) found last year.
"We propose that the essential building blocks within the stack are not individual spiral ramps but a 'parking garage' organized around two gently pitched ramps, one of which is the mirror image of the other — a dipole," said Guven, who was assisted in his research by one of his students, Dulce María Valencia. "This architecture minimizes energy and is consistent with the laminar structure of the stacks but is also stable."
In physics, these helical structures, which connect one layer of the ER with the next, are called defects. That word, Huber noted, carries no negative connotation in this context. "When you look at this through the eyes of physics, there are certain mechanisms that suggest themselves almost immediately," Huber said. "The edge of an ER sheet is a region of high curvature because the sheet turns around and bends. The bend is actually the thing that's forming the helix."
The bend creates a U shape that looks like half of a tube. Huber and his colleagues applied the principles of differential geometry to this curved membrane. Pulling the halves of a tube apart creates a flat region spanning the two U-shaped halves, which then become part of a sheet.
The geometrical idea is that one can actually get a sheet by pulling apart a network of tubes in a certain way," Huber explained. "Imagine that each of the U-shaped edges wants to bend, but when you try to connect those two U shapes together, each one is now bent. That's what the color figure is trying to show. A tube can generate a sheet if the edges come apart and they're allowed to bend in space."
According to Huber, this theoretical work provides a deeper story and richer vocabulary for discussing the shapes found in cell interiors. "One suspects that their shape is related to their function," he concluded. "In fact, scientists know that the shape of the ER can be an indicator of abnormal functions seen in certain diseases."
Julie Cohen | EurekAlert!
Staying in Shape
16.08.2018 | Max-Planck-Institut für molekulare Zellbiologie und Genetik
Chips, light and coding moves the front line in beating bacteria
16.08.2018 | Okinawa Institute of Science and Technology (OIST) Graduate University
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
Proteins must be folded correctly to fulfill their molecular functions in cells. Molecular assistants called chaperones help proteins exploit their inbuilt folding potential and reach the correct three-dimensional structure. Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. The chaperone TRiC uses a previously undescribed mechanism to perform actin folding. The study was recently published in the journal Cell.
Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle...
Scientists have discovered that the electrical resistance of a copper-oxide compound depends on the magnetic field in a very unusual way -- a finding that could help direct the search for materials that can perfectly conduct electricity at room temperatur
What happens when really powerful magnets--capable of producing magnetic fields nearly two million times stronger than Earth's--are applied to materials that...
08.08.2018 | Event News
27.07.2018 | Event News
25.07.2018 | Event News
16.08.2018 | Life Sciences
16.08.2018 | Earth Sciences
16.08.2018 | Life Sciences