Malfunction in spermatogenesis

The team of researchers working on Cylicins at UKB. (from left to right) Prof. Dr. Hubert Schorle, M. Sc. Andjela Kovacevic and Dr. Simon Schneider uncover the contribution of Cylicin Structural proteins in male infertility.
Foto: Rolf Müller / Universitätsklinikum Bonn (UKB)

Bonn researchers uncover contribution of Cylicin proteins to male fertility.

For successful fertilization, sperm should move forward rapidly and be shaped correctly. The unique structure of the sperm cells forms during spermiogenesis. Now, researchers from the University Hospital Bonn (UKB) and the Transdisciplinary Research Unit “Life & Health” at the University of Bonn have found that fertility problems in both mice and humans can be caused by loss of so-called cylicines. This causes defects in head and tail structure of sperm. The results of the study have now been published in the scientific journal “eLife”.

During spermiogenesis, round immature cells in the testes develop into mature sperm with a head and tail. At the front of the sperm head, which carries the genetic material, is the acrosome; a structure required to cross the protective layer around the egg during fertilization. The sperm tail allows the cell to actively reach the egg. The shape of the sperm is unique and the cytoskeleton contains special proteins found only in sperm cells. This cytoskeleton ensures the stable connection of the individual parts of the sperm. In the area of the sperm head there is the perinuclear theca (PT) which is a cytoskeletal structure surrounding the sperm head. In this PT Cylicin 1 and Cylicin 2 are located. The role of these proteins had not yet been investigated.

“Since cylicins are very similar in mice and humans, our study in mice also allows us to find out more about human sperm development,” says corresponding author Prof. Hubert Schorle from the Institute of Pathology at UKB, who is also a member of the Transdisciplinary Research Area (TRA) “Life & Health” at the University of Bonn. “We hoped to gain insights into the complex network of factors that control sperm development and thus also male fertility.”

Cylicines are a structural component of the sperm calyx

To find out where cylicins are needed in spermiogenesis, Prof. Schorle’s research group created and studied mice that can no longer produce Cylicin 1 and / or Cylicin 2. “Male mice lacking Cylicin 1 achieved fewer pregnancies and smaller litters when mated with healthy females,” says first author Dr. Simon Schneider of the Institute of Pathology and Bonn Technology Campus of the Faculty of Medicine. First author Andjela Kovacevic, a Ph. D. student in Prof. Schorle’s group, adds, “The absence of two or more copies of the Cylicins rendered male mice infertile: mating resulted in no pregnancies.” The reason for the observed infertility is that the sperm heads are smaller and the acrosomes are damaged. In addition, the tails are curled and wrapped around the sperm head, which massively restricts the mobility of the sperm. The Bonn researchers found that, an area of the PT called the calyx is missing in these sperm. “This suggests that the Cylicins are essential for this sperm structure” Prof. Schorle says.

Cylicines also regulate male reproduction in humans

The Bonn researchers also wanted to find out whether fertility problems in humans are also partly due to changes in the Cylicins. To this end, they teamed up with colleagues from the University of Münster. They were able to identify one patient in the patient cohort of the University Hospital Münster who has variants of Cylicin1 and Cylicin2 genes. His sperm has defects of the head and tail structure and the patient is unable to father a child. “Based on these results, testing for gene variants of Cylicins should be considered in cases of male infertility,” says Prof. Schorle, who also has a hopeful message for those affected: “Since ‘only’ the scaffolding of the sperm is damaged, an attempt to transplant the sperm head and thus the genetic material into an egg cell, might result in a successful fertilization.”

Simon Schneider, Andjela Kovacevic et al; Cylicins are a structural component of the 1 sperm calyx being indispensable for male fertility in mice and humans, eLife;

Press contact:
Dr. Inka Väth
Deputy Press Spokeswoman at the University Hospital Bonn (UKB)
Communications and Media Office at Bonn University Hospital
Phone: (+49) 228 287-10596

About the University Hospital Bonn: The UKB cares for about 500,000 patients per year, employs about 9,000 people and has a balance sheet total of 1.6 billion euros. In addition to the more than 3,300 medical and dental students, a further 585 people are trained in numerous healthcare professions each year. The UKB is ranked number one among university hospitals (UK) in NRW in the Science Ranking as well as in the Focus Clinic List and has the third highest case mix index (case severity index) in Germany. The F.A.Z. Institute has named the UKB Germany’s most sought-after employer and training champion among public hospitals in Germany in 2022 and 2023.

Wissenschaftliche Ansprechpartner:

Prof. Dr. rer.nat. Hubert Schorle
Institute of Pathology; Department of Developmental Pathology
University Hospital Bonn (UKB)
TRA “Life & Health”, University of Bonn
Phone: +49 228 287 16342


Simon Schneider, Andjela Kovacevic et al; Cylicins are a structural component of the 1 sperm calyx being indispensable for male fertility in mice and humans, eLife;

Media Contact

Dr. Inka Väth Kommunikation und Medien
Universitätsklinikum Bonn

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