Arsenic and Gold Clusters

Mention of arsenic poisoning usually brings to mind underhanded murder. However, the danger of arsenic poisoning from contaminated drinking water is far greater. Low concentrations of arsenic are found in nearly all soils and thus also in ground water.

About 140 million people worldwide possibly drink water that contains arsenic concentrations above the WHO-recommended limit of 10 ppb (parts per billion). Researchers at Jackson State University (MS, USA) have now developed a new approach for a rapid, easy, and highly sensitive arsenic test.

As Paresh Chandra Ray’s team reports in the journal Angewandte Chemie, their method is based on the aggregation of gold nanoparticles, and it selectively detects arsenic in drinking water down to concentrations of 3 ppt (parts per trillion).

Countries like India, Bangladesh, and Thailand are primarily affected by ground water with high arsenic concentrations. However, high concentrations of arsenic have also been found in some areas of North and South America. Once detected, the problem can fairly easily be addressed. Current analytical techniques are time-consuming and require a series of enrichment steps.

The new process could now speed up and simplify arsenic analysis. The scientists working with Ray have attached special organic molecules to the surfaces of gold nanoparticles. These molecules act as “ligands” for arsenic, meaning that they form a complex with it. Each arsenic ion can bind to three ligands, which allows it to link together up to three gold particles. The higher the arsenic concentration in the sample, the more strongly the gold particles clump together and the number of bigger aggregates increases. The color of gold nanoparticles in a liquid depends on their size. Whereas the arsenic-free gold nanoparticles appear red, arsenic-induced aggregation causes the color to change to blue. Concentrations down to 1 ppb can be detected with the naked eye by means of the color change. Arsenic binds to the ligands much more strongly than other metals; the researchers were able to increase this selectivity by attaching three different ligands to the gold.

One very precise method for detecting minimal changes in particle size is dynamic light scattering (DLS), in which laser light scattered by the particles is analyzed. By using DLS, Ray and his co-workers were able to detect and quantify arsenic concentrations as low as 3 ppt. In samples of well water from Bangladesh, the team found 28 ppb arsenic; in water from taps in Jackson (Mississippi, USA) they found 380 ppt.

Author: Paresh Chandra Ray, Jackson State University (USA), http://chem.jsums.edu/ray/

Title: Use of Gold Nanoparticles in a Simple Colorimetric and Ultrasensitive Dynamic Light Scattering Assay: Selective Detection of Arsenic in Groundwater

Angewandte Chemie International Edition 2009, 48, No. 51, 9668–9671, doi: 10.1002/anie.200903958